Activation of peroxisome proliferator activated receptor alpha ameliorates ethanol mediated liver fibrosis in mice
© Nan et al.; licensee BioMed Central Ltd. 2013
Received: 30 December 2012
Accepted: 31 January 2013
Published: 6 February 2013
Peroxisome proliferator activated receptor alpha (PPARα) ameliorates ethanol induced hepatic steatohepatitis. However, its role in alcoholic liver fibrosis has not been fully clarified. The aim of this study was to elucidate the effect and the molecular basis of PPARα in ethanol induced liver fibrosis in mice.
C57BL/6J mice were fed with 4% ethanol-containing Lieber-DeCarli liquid diet for eight weeks, and intraperitoneal injected with 5% carbon tetrachloride (CCl4) for the last four weeks to induce alcoholic liver fibrosis. PPARα agonist WY14643 was administered to mice during the last couple of weeks. The effects of PPARα induction on liver histology, activation of hepatic stellate cells (HSCs), as well as hepatic expression of inflammatory and fibrogenic factors were assessed.
The ethanol plus CCl4 treated mice exhibited progressive liver injury including piecemeal necrosis of hepatocytes, severe inflammatory cells infiltration and bridging fibrosis. This was accompanied by down-regulated hepatic expression of PPARα and the protective cytokines adiponectin, heme oxygenase-1 and interleukin-10. Additionally, up-regulation of the proinflammatory cytokine tumor necrosis factor-alpha, as well as the profibrogenic genes osteopontin, transforming growth factor-beta 1, visfatin, phosphatidylinositol 3-kinase, matrix metalloproteinase-2 (MMP-2) and MMP-9 was observed. WY14643 treatment restored expression of cytokines altered by ethanol plus CCl4 treatment and concomitantly ameliorated the liver injury.
The present study provides evidence for the protective role of PPARα induction in ameliorating ethanol mediated fibrosis through mediation of inflammatory and fibrogenic factors.
KeywordsPeroxisome proliferator activated receptor alpha Liver Ethanol Fibrosis Animal experiment
Alcoholic liver fibrosis is a severe form of alcoholic liver disease (ALD), which may progress to liver cirrhosis and hepatocellular carcinoma. Hepatic ethanol metabolism leads to the release of reactive oxygen species (ROS) and generation of lipid peroxidation products , which directly damage hepatocyte membranes and organelles, leading to inflammatory responses , including activation of Kupffer cells and subsequent release of proinflammatory and profibrogenic cytokines, such as tumor necrosis factor alpha (TNF-α) and transforming growth factor beta 1 (TGF-β1) . TGF-β1 plays a crucial role in the development of hepatic fibrosis , through transdifferentiating quiescent hepatic stellate cells (HSCs) into myofibroblast-like cells, suppressing degradation and stimulating production of extracellular matrix (ECM). It is of great practical significance to identify potent pharmacological agents which target the activated HSCs and fibrogenic mediators to protect against ethanol-related liver fibrosis.
Previously, we demonstrated the role of nuclear transcription factor peroxisome proliferators activated receptor alpha (PPARα) in lipid homeostasis and modulation of inflammatory responses in alcoholic steatohepatitis . Induction of PPARα significantly ameliorated the severity of ethanol induced liver injury by regulating expression of lipid metabolism and inflammation related genes. However, the role of PPARα in alcoholic liver fibrosis, the more severe form of ALD, remains largely unknown. In the present study, the underlying molecular mechanisms of ethanol induced hepatic fibrosis and the effect of PPARα in the pathogenesis of alcohol-induced liver fibrosis were elucidated.
Activation of PPARα by WY14643 lowered the serum alanine aminotransferase (ALT) and aspartic transaminase (AST) levels in mice under ethanol plus carbon tetrachloride (CCl4) treatment
Reversal of liver injury induced by ethanol and CCl4 after activation of PPARα
Effect of PPARα induction on activation of HSCs in ethanol and CCl4 induced liver fibrosis in mice
We evaluated the role of PPARα in the development of liver fibrosis by assessing the hepatic expression of α-smooth muscle actin (α-SMA), a well-known marker of activated HSCs. Compared with control or ethanol treated mice, markedly increased α-SMA expression in the activated HSCs and fibrotic areas of liver sections was observed in ethanol plus CCl4 treated mice, which was significantly blunted by WY14643 treatment (Figure 2C).
Hepatic PPARα expression in mice fed with ethanol liquid diet and/or intraperitoneally injected with CCl4, and treated with WY14643
Induction of PPARα regulated hepatic expression of inflammatory cytokines
Activation of PPARα suppressed hepatic expression of profibrogenic cytokines
Activation of PPARα enhanced hepatic expression of anti-fibrotic cytokines
Alcoholic liver fibrosis is characterized by severe liver inflammatory response and fibrosis owing to augmented oxidative stress as well as generation of cell-toxic and profibrogenic ethanol metabolites, such as acetaldehyde and lipid oxidation products, which cause hepatocellular injury and activation of HSCs. A representative animal model of alcoholic liver fibrosis should reflect the characteristic metabolic changes and typical histological lesions of progressive fibrosing steatohepatitis, enabling ascertainment of pathogenesis and evaluation of drug therapy. In the present study, we established an experimental model of alcoholic liver fibrosis by feeding C57BL/6J mice with 4% ethanol-containing Lieber-DeCarli liquid diet for 8 weeks and combined with 5% CCl4 intraperitoneal injection for the last 4 weeks. After the combined administration, the mice rapidly and consistently developed alcoholic liver fibrosis manifested histologically by pronounced inflammatory infiltration, piecemeal hepatocellular necrosis, perisinusoidal and bridging fibrosis, together with enhanced hepatic expression of α-SMA, as well as significantly elevated serum ALT and AST levels. Conversely, ethanol treatment caused moderate macrosteatosis and mild inflammatory infiltration in the mice liver. These findings indicated that an alcoholic liver fibrosis model could be established rapidly and successfully by feeding mice ethanol liquid diet combined with a small amount of CCl4 intraperitoneal injection. Ethanol was the key mediator to induce liver injury, whilst CCl4 accelerated the progression of liver injury by increasing lipid accumulation [6, 7] and enhancing oxidative stress [8, 9].
With the progression of liver fibrosis, hepatic PPARα expression was reduced, suggesting that abnormal expression and/or dysfunction of PPARα might be involved in the development of ethanol plus CCl4 induced liver injury. We further demonstrated that induction of PPARα by specific agonist WY14643 administration for two weeks prominently attenuated liver injury, as evidenced by decreased serum ALT and AST levels, diminished inflammatory response, reduced collagen deposition, as well as suppressed activation of HSCs. These results indicated that PPARα played an important protective role in the progression of alcoholic liver fibrosis.
Oxidative stress and release of inflammatory cytokines induced by ethanol metabolism can evoke the activation of HSCs. Once activated, HSCs migrate to the site of liver injury and secrete excessive ECM , which is the pivotal event triggering the process of liver fibrogenesis. A variety of proinflammatory and profibrogenic factors are involved in controlling the activation and proliferation of HSCs. TNF-α, a pivotal inflammatory cytokine, exerts a profibrogenic function by inducing HSCs activation and inhibiting HSCs apoptosis . We found that hepatic expression of TNF-α was increased in mice fed with ethanol liquid diet and intraperitoneally injected with CCl4, which was significantly reduced by WY14643 treatment. In addition, we demonstrated that hepatic expression of OPN and TGF-β1 was up-regulated by ethanol with or without CCl4 treatment and restored by WY14643 administration. OPN is a chemoattractant molecule engaged in the hepatic inflammatory response by promoting neutrophil infiltration in the liver . As a biomarker of fibrosis , OPN can bind to integrin on the surface of HSCs to drive the fibrogenic response by regulating collagen I deposition [14, 15]. TGF-β1 is the most critical profibrogenic factor involved in the initiation and maintenance of liver fibrogenesis [4, 16]. It was reported that TGF-β1 increased expression of OPN , which in turn up-regulated TGF-β1 expression and activated HSCs through TGF-β1/Smad pathway , forming a vicious circle promoting liver fibrogenesis. Thus, the role of PPARα induction in alleviating alcoholic fibrotic hepatitis was possibly related to the down-regulation of TNF-α, OPN and TGF-β1, as well as the subsequent suppression of inflammatory response and fibrogenesis.
To further clarify the mechanism by which PPARα alleviated ethanol mediated liver fibrosis in mice, hepatic expression of visfatin, a novel identified adipocytokine, was assessed. It was considered as a proinflammatory cytokine and could be up-regulated by TNF-α [19, 20]. In the livers of ethanol plus CCl4 treated mice, visfatin expression was markedly up-regulated, accompanied with increased hepatic expression of PI3K, MMP-2 and MMP-9. In accord with our results, one study reported that visfatin induced MMP-2 and MMP-9 production in endothelial cells, which was mediated by the PI3K signaling pathway . MMP-2, secreted by activated HSCs, is considered a profibrotic mediator exerting proliferation and migration of HSCs [22, 23], and can be up-regulated by TGF-β and/or ROS stimulation [24, 25]. Meanwhile, MMP-9 can stimulate HSCs activation and up-regulate TGF-β1 expression [26, 27], which in turn induces MMP-9 expression through PI3K/Akt/nuclear factor-kappa B signaling pathway . We demonstrated that PPARα agonist significantly repressed hepatic expression of visfatin, and consequently reduced PI3K, MMP-2 and MMP-9 expression, thus suppressing the progression of ethanol mediated liver fibrosis.
We considered whether protective cytokines mediated the effect of PPARα in alleviating ethanol induced liver injury. Indeed, we found that reduced hepatic expression of adiponectin in alcoholic liver fibrosis mice was restored by WY14643 treatment. Adiponectin exerts its anti-inflammatory property through suppressing TNF-α production and secretion , and alleviates hepatic fibrosis by maintaining quiescence of HSCs and inducting apoptosis of activated HSCs [30–32]. Adiponectin induces expression of several other protective mediators, and an IL-10/HO-1 pathway is involved in the anti-inflammatory effects of adiponectin . IL-10 inhibits intrahepatic fibrogenesis by suppressing production of collagen I [34, 35], down-regulating expression of profibrogenic factors TGF-β1, MMP-9 and TNF-α [36–38], and promoting apoptosis of activated HSCs . In addition, IL-10 up-regulates HO-1 expression through the p38 mitogen-activated protein kinase pathway [40, 41]. HO-1 acts as an anti-oxidant and anti-fibrogenic protein in the liver. It was reported that induction of HO-1 suppressed oxidative stress and HSCs activation, thus inhibiting liver fibrogenesis in nutritional fibrotic steatohepatitis in mice . In keeping with this observation, we found that IL-10 and HO-1 expression was up-regulated by WY14643, together with the improved liver injury induced by ethanol plus CCl4 administration. Therefore, the protective role of PPARα induction against liver inflammation and fibrosis was mediated by up-regulating anti-inflammatory and anti-fibrogenic cytokines.
In summary, the present study demonstrated a protective role of PPARα induction in experimental alcoholic liver fibrosis through down-regulating the proinflammatory and profibrogenic factors TNF-α, OPN, TGF-β1, visfatin, PI3K, MMP-2 and MMP-9, and up-regulating the tissue-protective cytokines adiponectin, IL-10 and HO-1. Consequently, PPARα agonist administration might serve as an effective therapeutic strategy for alcoholic liver fibrosis.
Materials and methods
Animals and treatments
Serum ALT and AST levels were measured by the enzymatic method using an automatic biochemical analyzer (Olympus UA2700, Japan) according to the manufacturer’s instructions.
Haematoxylin and eosin stained paraffin-embedded liver sections (5 μm thick) were scored as follows: (a) degree of steatosis (0 ≤ 10%, 1 = 10-33%, 2 = 33-66%, 3 ≥ 66%); (b) degree of necroinflammation (0 = none, 1 = mild, 2 = moderate, 3 = severe); (c) stage of fibrosis (0 = no fibrosis, 1 = mild/moderate zone 3 perisinusoidal fibrosis or portal fibrosis only, 2 = zone 3 and portal/periportal fibrosis, 3 = bridging fibrosis, and 4 = cirrhosis) in accordance with a scoring system for ALD designed by Dominguez et al.  and the Chinese Guidelines for the diagnosis and management of alcoholic liver disease .
Immunostaining was performed in paraffin-embedded liver sections using the specific antibody and an avidin-biotin complex (ABC) immunoperoxidase method. Briefly, after antigen repair, the primary specific antibody for α-SMA (dilution 1: 200) (Santa Cruz Biotechnology, Santa Cruz, CA) was applied. The primary antibody was omitted and phosphate buffered saline was used as the negative control. After extensive rinsing, the biotinylated secondary antibody and ABC complex/horseradish peroxidase were applied. Peroxidase activity was visualized by applying diaminobenzidine (Santa Cruz Biotechnology) to the sections, which were then counter-stained with hematoxylin. Quantitative analysis of α-SMA stained liver sections (200 fold) was performed by morphometric analysis: the average area density (areas of positive cells/total areas × 100%) in each section was estimated.
Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) analysis of hepatic mRNA expression
Primers used for quantitative RT-PCR analysis
F 5’-GATGTCACACAATGCAATTCG -3’
R 5’-GGTAGGCTTCGTGGATTCTCT -3’
R 5’ -ACTGCCGTACAACTCCAGTGAC-3’
F 5’- GGAAAGACCATGAGAAAGATGC-3’
F 5’- GCACGGCGATTACACTCTTAC-3’
F 5’- TTGTGCTGAAAGATACCCTCAA-3’
Western blot analysis of hepatic protein expression
Total protein was extracted and concentration was measured by the Bradford method (DC protein assay; Bio-Rad, Hercules, CA). Equal amounts of protein (100 ìg/well) were loaded onto 12% SDS-PAGE for each sample and proteins were transferred onto equilibrated polyvinylidene difluoride membranes (Millipore Corporation, Billerica, MA) by electroblotting. The membranes were incubated with primary antibodies of PPARα, OPN, TGF-β1, visfatin, PI3K, MMP-2, MMP-9, adiponectin, HO-1 and â-actin (Santa Cruz Biotechnology), respectively, overnight at 4°C. Membranes were further incubated with secondary antibody for 1h at room temperature. Proteins were detected by enhanced chemiluminescence (Santa Cruz Biotechnology). The amount of protein expression was corrected by that of â-actin in the same sample and the bands were quantified by scanning densitometry using the digital Kodak Gel Logic 200 (Carestream Molecular Imaging, Woodbridge, CT).
All data are expressed as mean ± standard deviation (SD). Statistical analysis on the data was performed by one-way analysis of variance (ANOVA) or Kruskal-Wallis H test, with the least significant difference-t (LSD-t) test or Mann–Whitney u test for post-hoc comparison using SPSS 13.0 (v. 13.0; SPSS Inc., Chicago, IL), and P- value below 0.05 was considered significant.
Peroxisome proliferator activated receptor alpha
Hepatic stellate cells
Alcoholic liver disease
Tumor necrosis factor-alpha
Transforming growth factor-beta1
Alpha-smooth muscle actin
This work was funded by Wang Bao-en Foundation of Hepatic Fibrosis, Chinese foundation for hepatitis prevention and control, No. 2009009.
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