Impact of extruded flaxseed meal supplemented diet on growth performance, oxidative stability and quality of broiler meat and meat products
© Anjum et al.; licensee BioMed Central Ltd. 2013
Received: 15 December 2012
Accepted: 31 January 2013
Published: 8 February 2013
This study was intended to explore the effect of extruded flaxseed meal supplemented diet on broiler growth performance, oxidative stability and organoleptic characteristics of broiler meat and meat products. 120 (day old) broiler chicks were randomly allotted to 12 experimental groups and fed on diets containing extruded flaxseed meal at 0, 5, 10 and 15%. The supplementation of extruded flaxseed in the diet decreases the body weight gain, feed intake and increased feed conversion ratio (FCR) values of broilers. The antioxidant enzymes were strongly influenced by different levels of extruded flaxseed supplementation among treatments. The TBARS assay revealed that maximum malondialdehyde were produced in T3 containing highest extruded flaxseed level (15%) and minimum malondialdehyde were produced in T0 treatment having no extruded flaxseed. The TBARS values ranged from 0.850-2.106 and 0.460-1.052 in leg and breast met respectively. The Free radical scavenging activity varied significantly and DPPH values of breast meat ranged from 20.70% to 39.09% and in leg meat 23.53% to 43.09% respectively. The sensory acceptability of broiler meat nuggets was decreased with the increase in the level of flaxseeds due to the lipid peroxidation of polyunsaturated fatty acids (PUFA) which generated off flavors and bad odors. Feeding extruded flaxseed to chicken through feed strongly inflated the quality and functional properties, fatty acid contents and reduced the oxidative stability of broiler meat and meat products. The present study concludes that up to 10% of flaxseed meal may be used in broiler diet to enhance the omega 3 fatty acids content in the broiler meat.
KeywordsBroiler meat Extrusion Flaxseed Nuggets PUFA Lipid stability
Flaxseed is among unique oil seed crops because of its exceptionally high content of α-linolenic acid (ALA), contains 35 to 45% oil, of which 45 to 52% is α-linolenic aci . Flaxseed (Linum Usitatissimum L.) is also one of the oldest domesticated crop that is under cultivation in Europe and Asia since dating back thousands of years (3000–5000 BC). Approximately more than two hundred species of flaxseed have been recognized . Flaxseed is grown as oil crop, as fiber crop and oil is extracted from oilseed varieties. A large proportion of flaxseed comprises nutritional components such as oil, soluble fiber, protein, lignans, vitamins and minerals .
Nutritionists during last two decades are actually clear about selected foods that play an important role in maintaining physical and mental health status of consumers. Beyond meeting nutrition needs, it is generally recognized that dietary factors are considered to modulate detrimental development of some chronic diseases. With increased consumption of highly saturated fat foods, it seems feasible that modern diets do not meet healthy eating guidelines and deficient in certain long chain omega-3 fatty acids. It is widely accepted that inadequate intakes of omega-3 polyunsaturated fatty acids (PUFA), principally alpha-linolenic acid (ALA) and docosahexaenoic acid (DHA), adversely affect cardiovascular function . Flaxseed is rich source of omega-3 polyunsaturated fatty acids and it is helpful in prevention of cardiovascular diseases and cancer. The flaxseed contains all essential amino acid of the protein. It is an excellent source of fiber, lecithin, vitamins and minerals. Moreover, flaxseed is of fastidious significance for its role in lowering the risk of breast and colon cancers . A large proportion of flaxseed comprises nutritional valuable components such as protein (200-240 g/kg), dietary fiber (250–280 g/kg) and flax oil (350-450 g/kg).
The health benefits are related with the ingestion of polyunsaturated fatty acids (PUFA) and dietary fiber. The flaxseed oil contains almost 50% essential fatty acid like alpha-linolenic acid of the total fatty acids that play a significant role in reducing the risk of coronary heart diseases . However, the drawbacks with the dietary inclusion of flaxseed are the presence of anti-nutritinal factors, which may limits its utilization in poultry diets. Mucilage coming from hull, linatin dipeptide (vitmine B6 antagonistic), cyanogenic glycosides, trypsin inhibitor and phytic acid are the most important antinutritional factors found in flaxseed .
Alpha-linolenic acid (ALA) and linoleic acid (LA) are the essential fatty acids among the polyunsaturated fatty acids. Human body cannot synthesize alpha-linolenic acid linoleic acid so they should be present in the diet. LA and LNA are metabolized to long chain PUFA in mammalian cells. The omega-6 fatty acid LA is converted to γ-LNA and diomo-γ-LNA by desaturase and elongase enzymes to produce arachidonic acid (AA). AA is further metabolized to eicosanoids or docosapentaenoic acid (DPA). LNA which is omega-3 fatty acid is converted to stearidonic acid and eicosatetraenoic acid to synthesize eicosapentaenoic acid (EPA) by the same enzymes used in synthesis of AA. Eicosapentaenoic acid is then metabolized to docosahexaenoic acid (DHA) or eicosanoid .
The limiting consumption of flaxseed is due to presence of anti-nutritional factors such as cyanogenic compounds and tannin which negatively influence the health and well-beings of the masses. The cyanogenic glycosides (CG) are amino acids derived from flaxseed constituents which release a potent cell respiration inhibitor; toxic hydrogen cyanide (HCN) via hydrolysis. The presence of these compounds restricts flaxseed applications in feed and foodstuff by producing bitterness in final products . Presence of phytic acid in the diet reduces micronutrient bioavailability and metabolizeable energy which ultimate lead towards poor growth of poultry birds. Anti-vitamin B6 compound named as ''linatine'' reduces dry matter and produces vitamin B6 deficiency. Omega-3 enriched meat has several health benefits. It plays an important role in prevention or treatment of cardiovascular disease, hypertension, arthrosclerosis, cancer neurological disorders and inflammatory disease. The increased intake of omega-3 fatty acids decreases serum cholesterol which beneficially affects blood pressure, skin diseases, thrombosis atherosclerosis and diabetes, arterial compliance and hyperlipidemia response .
Extrusion cooking is highly adaptable and focused form of processing in which food or feedstuff is enforced to flow under controlled conditions of heating and shearing through a terminal die. Extrusion processing of feed ingredients has become very popular during the last two decades. The principal features of extrusion cooking include flexible product characteristics, high energy efficiency, less space required for operation, new feed products formulations, automated control system with high productivity and product quality with no effluent showing environmental-friendly technology to be used. Optimized results for temperature (146.0°C), feeding rate (32.7 kgh−1), screw speed (152.5rpm), moisture content (12.5%) and the hydrocyanic acid removal rate (93.23%) were founded during the extrusion detoxification technique on flaxseed via stepwise non-linear regression analysis and response surface method. It has been demonstrated that maximum removal rate of mucilage (60.3%) from flaxseed meal can be achieved at different co-rotating twin-screw extruder die temperatures (80-160°C), screw speeds (300-900rpm) and initial moisture contents ranged as 18.8-35.1% . There exist a relationship between the stability of the flaxseed derived protein and antifungal activity with temperature variable (50-90°C) using response surface methodology . Regression analyses recommended the significant negative consequences on the residual antifungal activity against all test fungi using treatment variable. The protein content exits in flaxseed by means of response methodology with 5 central points and 4 axial points for independent variables . Extrusion cooking improved the degradation of flaxseed mucilage and negatively influences the compact fiber structure flow behavior index with the addition of initial moisture content, high temperature and decreased screw speed .
Incorporation of n-3 fatty acids from flaxseed into eggs and meat is an efficient process tool to increase the polyunsaturated fatty acids content of meat and eggs. The chicken is able to digest flaxseed in the digestive tract, absorb nutrients from flaxseed in the small intestine, and then further metabolize some of the alpha-linolenic acid (ALA; C18:3n-3), elongating and desaturating the ALA to eicosapentaenoic acid (EPA; C20:5n-3) and docosahexaenoic acid (DHA; C22:6n-3) for deposition in lipid stores such as egg yolk, and phospholipids in meat products. Flaxseed is a valuable and natural source of n-3 fatty acids for the poultry producer wanting to market an n-3 polyunsaturated fatty acid (PUFA) enriched poultry product. 300 mg of omega-3fatty acids per 100 g of breast meat can be achieved in 26.2 days at 10% ground flaxseed meal . Keeping in view the breakthrough results in flaxseed or flaxseed meal studies, the present study was carried out to compare the effects of extruded flaxseed meal supplementation on broiler growth performance, oxidative stability, ALA, polyunsaturated fatty acids content, quality attributes of broiler meat and sensory acceptability of meat products.
Materials and methods
Composition of diet
The composition of the control feed was 300 g of corn, 200.0 g of soya been meal of 44% protein, 140.0 g of Sorghum, 100.0 g of sunflower meal, 60g of barley, 50 g of rye, 50.0 g of beef tallow, 20 g of dicalcium phosphate, 2 g of sodium bicarbonate, 10 g of salt and 10.0 g of Sepiolite per kg of feed. The analysis of feed revealed that metabolizable energy of the control feed was 2850 kcal/kg, crude protein (16.0%), crude fat (4.1%) fiber (5.1%) and ash content was (7.4%). Additionally flaxseed was supplemented in the feed at a level of 5, 10 and 15% as mentioned in the treatment plan (Table 1).
Extrusion of flaxseed
Hammered milled flaxseed was extruded using the single screw extruder, Extru-tech E325 (Extru-tech, Sabetha, Kansas). Designed single screw extruder was consisting of feed delivery system, heating arrangement, preconditioning system, power transmission system and extruder barrel. Optimum pre-conditioning was carried out by the addition of steam in the pre-conditioner part of extruder prior extrusion heating of flaxseed. The conditions for extrusion of flaxseed were extruder final head temperature (80-120°C), extruder barrel speed (80-120rpm) and feeder speed (12-16rpm) as described by . The extruded flaxseed was then added to broiler diet.
Experimental birds management
One day old (120) broiler chicks of Hubbard strain were purchased from local market. The chicks were weighed individually and then randomly divided in to 12 experimental units of 10 birds each. These units were further allotted to 4 treatments in such a way that each treatment has 3 replicates. The birds were kept in a student reserved research room having 31 pens of 12 sq feet capacity each. The room and the pens were cleaned thoroughly, white washed and then disinfected with formalin with a ratio 1:12 inside and outside the pen before the start of experiment. A layer of saw dust was used as litter in each pen that was stirred regularly during the experiment to keep it in dry condition. The temperature of the experimental room was maintained at 95°F during the first week. It was then lowered by 5°F till it reached 75 F° ±2. Twenty four hour light and proper ventilation was provided in the experimental room throughout the experimental period. Feed along with fresh and clean water was given ad-libitum. The experimental birds were reared up to six weeks. First two weeks, the chicks were fed on the control feed and after the four weeks the feed was supplemented with extruded flaxseed.
Treatment plan of experimental birds
Control + 5% extruded flaxseed
Control + 10% extruded flaxseed
Control + 15% extruded flaxseed
Analysis of broiler meat
Sample preparation for analysis
5 gram meat sample was taken in 50 ml polypropylene tube having a cap and sample homogenized by using phosphate buffer and glycerol (20%) pH (7.4) with the help of homogenizer. During the process of homogenization, tubes were placed in ice cold water to dissipate the heat. Filtration process was done to remove connective tissues from sample by using muslin cloth.
The superoxide dismutase (SOD) activity was determined by measuring the ability of enzyme to inhibit cytochrome ‘c’ oxidation . The catalase (CAT) activity was measured by observing decomposition of hydrogen peroxide  and one international unit (IU) was equivalent to one mmole H2O2 consumed/min/mg protein. The Glutathione peroxidase (GPX) activity was estimated by using the method of  and activity was measured as nmol of NADPH/min/mg Protein. The Glutathione reductase (GR) activity was assayed by following the reduction of glutathione .
1, 1-diphenyl-2-picrylhdrazyl (DPPH) scavenging activity
The free radical scavenging activity of leg and breast meat was determined by following modified method of . Meat sample having protein concentration (1mg/1mL) took 100 μL fraction volumes in a test tube. Freshly prepared 0.0012M DPPH (1, 1-diphenyl-2- picrylhydrazyl) in methanol solution was added 2 mL in tubes containing 100 μL volume of sample. The reaction was incubating for 30 minute at room temperature in dark place. Absorbance of resultant mixture was recorded at 517 nm against blank and control using UV/Vis. spectrophotometer.
Thiobarbituric acid reactive substances (TBARS) assay
Fatty acids profile of broiler meat
The polyunsaturated fatty acid content of omega three enriched broiler meat was determined by using gas liquid chromatography . Lipids from meat samples were extracted using a non-polar solvent, n-hexane (GC-grade). The fatty acids profile of extracted lipids were prepared and analyzed according to the AOCS (1998) Method No. Ce 1f-96. 50 μl oil sample was methyated in the presence of 4 mL KOH (1 M) at room temperature for one hour in order to produce fatty acids methyl esters. The resultant methyl esters were extracted with GC grade n-hexane for immediately analyzing by Gas Chromatograph (Agilent Technologies, 6890N) equipped with an auto sampler, flame-ionization detector (FID) and fused capillary column (Silica 30m × 0.25 film thickness) apparatus. Samples (1 μL) were injected with Nitrogen (3.5 mL/min) as a carrier gas onto the column, which was programmed for operating conditions such as column oven temperature 220°C for 7.5 minutes, split ratio (50%) with injector and detector temperatures (260°C). Peak areas and total fatty acids profile percentages were calculated for each sample by retention time using Agilent Chem. Station software. The standards of fatty acids methyl esters purchased from Sigma-Aldrich were also run under the same conditions for comparison with experimental samples.
Preparation of nuggets
The nuggets of omega 3 enriched broiler meat were prepared by the method described by . The basic recipe of the nuggets is as Chicken boneless 500 g, Egg 1, oil as required for frying, Black pepper 12 g, Garlic paste 10 g, Onion 100 g, Plain flour 120 g, Bread crumbs 70 g, salt 20 g. Hand deboned chicken breasts and leg meat was separated and utilized for the development of the nuggets. Breast and leg meat was minced separately with respective additives as mentioned in the recipe of nuggets. All ingredients were mixed and then uniformly blended to obtain a uniform mixture. Then sheeting was done and nuggets were shaped into 30 mm diameter. After nuggets were prepared, they were dipped in plain flour and bread crumbs separately. The frying of the nuggets was done in canola oil at 180°C till golden brown color was appeared.
Physico-chemical analysis of nuggets
The pH of Nuggets was measured by using pH meter by following the method as described by . The colour of nuggets was measured by using a hand held tristimulus colorimeter (Color Test Meter II) at regular storage intervals (0,10,20,30 days) as described by . The water activity of Nuggets was determined by using an electronic Hygropalm water activity meter (Model Aw-Win, Rotronic, equipped with a Karl-Fast probe) as described by  The textural characteristics of sausages were analyzed at different storage intervals by means of texture analyzer (Mod. TA-XT2, Stable Microsystems, surrey, UK) Texture was determined with the help of texture analyzer (model TA_XT Plus, Stable Microsystems, Surrey, UK) as described by .
Sensory evaluation of nuggets
The sensory evaluation of fried nuggets was carried out for the different attributes like color, appearance, taste, texture and off flavor production by using nine point hedonic scale after 0, 10, 20 and 30 days, by using the method as described  by trained panelists as the method described.
The data obtained for each parameter was subjected to statistical analysis to determine the level of significance by using the software package (Statistic 8.1) according to the method described . The Duncan’s multiple range (DMR) test was used to estimate the level of significance that existed between the mean values.
Results and discussion
Body weight gain (g/week)) and feed conversion ratio of the broiler birds fed on extruded flaxseed on weekly basis
94.0±3 (BWG) 1.71±0.04 (FCR)
Enzyme assay of broiler blood
Antioxidant enzymes of blood serum of broiler birds fed on different levels of extruded flaxseed through feed
SOD (50% Pyrogyllol auto-oxidation/min/mg)
CAT (mmol of H2O2 decomposed/min/mg)
GSH-Px activity (nmol NADPH/min/mg Protein)
Glutathione reductase (u/g protein)
Oxidative stability of broiler meat
Thiobarbituric acid reactive substances (TBARS) assay
Total Lipids, TBARS assay, Free radical scavenging activity of leg and breast meat of broilers fed on extruded flaxseed meal supplemented diet
Total lipids (%)
TBARS assay (nmol of malondialdehyde/mg of meat)
Free radical scavenging activity (%)
Total lipids (%)
TBA assay (nmol of malondialdehyde/mg of meat)
Free radical scavenging activity (%)
Fatty acid analysis of broiler meat
Fatty acid profile of breast and leg meat of broilers fed on extruded flaxseed meal supplemented diet
Physico-chemical analysis of nuggets
pH, water activity, color and texture values of nuggets of leg and breast meat of broilers fed on extruded flaxseed meal supplemented diet
Breast meat nuggets
Leg meat nuggets
107.50± 3 C
Water activity (aw) is the most important property of water in food system. Scott (1957) reported that there is an optimum water activity level below that the micro organism cannot grow well. The results showed that the different treatments of extruded flaxseed have significant effect on water activity of leg meat nuggets. Water activity of leg meat nuggets ranged from 0.683-0.676. During storage the water activity of leg mat nuggets decreased as the time period increased. At 0 day all the treatments have higher water activity as compared to the 10, 20 and 30 days. The lowest water activity was observed at 30 in all treatments. Water activity of breast meat nuggets ranged from 0.671-0.656. During storage the breast meat nuggets water activity behavior was same as that of leg mat nuggets. The water activity decreased with the passage of time during storage and minimum water activity was observed at day 30. The results of my study are in contrast with the findings of  who observed no change in water activity in omega enriched sausage during storage. This variation in results may be due to the different level of enrichment or different storage conditions.
Food appearance is one of the major factors that determine the consumer acceptance and ultimately the sale of product. Food quality and shelf life is limited by the oxidation of polyunsaturated fatty acids and bacterial contamination. The results revealed a highly significant effect of different treatment of extruded flaxseed and storage days on color of the broiler meat nuggets. However the interactive effect of treatments and storage days was significant. The color of leg meat nuggets ranged from 115–107. During storage the values for the color decreased with the passage of time. The decrease in value indicated that the nuggets become darker during storage. In T3 minimum value 90 was observed at day 30. The color values decreased in all the treatments of extruded flaxseed during storage. The color values for breast meat nuggets fanged from 125–119. During storage the breast meat nuggets showed the same behavior as the leg meat nuggets. The color values tend to decrease with the storage days increase. The values for color decreased from 0 day to 30 days to the lowest level in all the treatments of extruded flaxseed. The results of my study are in close collaboration with the finding of , who studied the color characteristics of omega enriched sausages and found that the color become dark with the passage of time.
Texture is an important tool to check the acceptability of meat products during its storage period. The texture values of leg meat nuggets ranged from 969.3-974.8. During storage the texture values increased with the increase in storage days. The texture value in all the treatments ranged from 969.3 to 974.8 at 0 day which tend to increase 978.3-980.0 at day 30. During storage the breast meat nuggets followed the same pattern as leg meat nuggets. The texture values tend to increase with the storage days. At 0 days the texture values for all the treatments ranged 979.37-985.47 which increased to 991.10-994.33 at day 30. The increase in hardness may be associated with the decrease in water activity of the nuggets with the advancement of storage period. The results of present study are in close collaboration with  who stated that the texture value varied significantly with the advancement of storage period.
Sensory evaluation of nuggets
The development of new food products by modification of ingredients or processing conditions, cost reduction and quality control, often employs sensory evaluation techniques to determine the acceptability of food .
Aroma, flavor, taste and overall acceptability of nuggets of leg and breast meat of broilers fed on Extruded Flaxseed
Breast meat nuggets
Leg meat nuggets
6± 0.40 BC
7± 0.40 A
Flavor is an important parameter in sensory evaluation of a food product; it is the combined perception of smell, taste and mouth feel, making attraction for the consumers. It was obvious from the results that the treatments and storage days had significant effect on the flavor of nuggets; however the interactive effect of treatments and storage days was not significant. The nuggets from the control treatments obtained highest score for the flavor in all the treatments. The flavor values tend to decrease as the level of extruded flaxseed increased. During storage flavor value decreased with the storage days. The minimum value for flavor was observed in T3 (5.25) at day 30. The decrease in flavor value is due to the peroxidation of PUFA which results in off flavors and bad odors .
Taste is a very important attribute for the acceptance of a food product. The results showed that there was significant effect of treatments and storage days on taste of nuggets. The mean values revealed that T0 and T1 were much liked by the judges and got highest score,  and T3 got minimum score  at 0 day. During storage the score for taste of nuggets decreased and minimum score was obtained by T3 at day 30. The decreasing trend in taste of nuggets may be associated with the peroxidation of poly unsaturated fatty acids.
Overall acceptability is another important parameter in sensory evaluation of a product. It determines on the basis of quality scores obtained from aroma, taste, and flavor. The results showed that overall acceptability of nuggets was significantly affected by different treatments while the storage affect was non-significant. T0 was highly acceptable by the panelists as got highest score for overall acceptability  while T3 got minimum score  for the overall acceptability at day 30. The results of my study are in agreement with  who stated that the overall acceptability decrease with increase in omega enrichment in meat products.
The present study was designed to investigate the relationship of various quality attributes and storage stability of broiler meat and meat products with different concentrations of extruded flaxseed fed to the broiler birds. The supplementation of extruded flaxseed in the diet of broiler birds resulted decrease in the body weight, feed intake and higher FCR values. T3 (15% extruded flaxseed) achieved lowest weight and feed intake. The antioxidant enzyme assay resulted in significant difference among treatments. The chemical analysis of extruded flaxseed fed meat revealed that the fat contents of leg meat greatly increased as compared to breast meat. Maximum fat contents were observed in T3 (15% extruded flaxseed). Thiobarbituric acid reactive substances (TBARS) were evaluated in the form of n-moles of malondialdehyde by thiobarbituric acid test which showed the lipid oxidation stability of meat. T3 showed the highest amount of n-mole of malondialdehyde which revealed that this group has lowest lipid stability than other groups in this study. Free radical scavenging activity improved in meat by supplementation of higher concentration of extruded flaxseed in diet. The results showed the maximum free radical scavenging activity was found in T3. Fatty acid profile was determined by Gas Chromatography. The results showed higher omega-3 contents in higher level of extruded flaxseed fed meat. Omega-3 fatty acids were deposited according to dose supplemented in the meat. The maximum omega-3 contents were observed in T3 having highest level of extruded flaxseed (15%) than all the other treatments of study. The results from storage study revealed the significant effect of storage on the physicochemical and sensory properties of omega enriched meat nuggets. In the present study it is concluded that as the concentration omega-3 fatty acids increased in broiler meat with the higher level of extruded flaxseed supplementation in diet. Extruded flaxseed supplemented feed increased the total fat contents but decreased the oxidative stability of omega-3 enriched meat. High levels of extruded flaxseed negatively affected the physicochemical and sensory properties of omega-3 enriched nuggets during storage.
The present study concluded that as the concentration of omega-3 fatty acids increased in broiler meat with the higher level of extruded flaxseed supplementation in diet. Extruded flaxseed supplemented feed increased the total fat contents but decreased the oxidative stability of omega-3 enriched meat. High levels of extruded flaxseed negatively affected the physicochemical and sensory properties of omega-3 enriched nuggets during storage.
Thiobarbituric Acid Reactive Oxygen Species
High-Performance Liquid Chromatography
Poly Unsaturated Fatty Acids.
The authors wish to express sincere thanks to National Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan for providing financial assistance to carry out this research work.
- Bhatty RS: Nutritional composition of whole flaxseed and flaxseed meal. Flaxseed in human nutrition. Edited by: Cunnane SC, Thompson LH. 1995, AOCS Press, Champaign, IL.Google Scholar
- Carter JF: Potential of flaxseed and flaxseed oil in baked goods and other products in human nutrition. Cereal Foods World. 1993, 38 (10): 753-759.Google Scholar
- Diederichsen A, Richards KW: Cultivated flax and the genus linum L.-taxonomy and germplasm conservation. Flax, the genus linum. Edited by: Muir A, Westscott N. 2003, 22-54. Taylor & Francis, London, UK.Google Scholar
- Palmquist DL: Omega-3 fatty acids in metabolism, health, and nutrition and for modified animal product foods. Prof l Animal Sci. 2009, 25: 207-249.Google Scholar
- Oomah BD, Mazza G: Flaxseed proteins – a review. Food Chem. 1993, 48: 109-114. 10.1016/0308-8146(93)90043-F. 10.1016/0308-8146(93)90043-FView ArticleGoogle Scholar
- Dale N, Batal A: Feedstuffs reference issue and buyers guide. Feedstuffs. 2008, 79: 17-20.Google Scholar
- Schmitz G, Ecker J: The opposing effects of n-3 and n-6 fatty acids. J of Lipid Res. 2008, 47: 147-155. 10.1016/j.plipres.2007.12.004. 10.1016/j.plipres.2007.12.004View ArticleGoogle Scholar
- Mukhopadhyay N, Sarkar S, Bandyopadhyay S: Effect of extrusion cooking on anti-nutritional factor tannin in linseed (linum usitatissimum) meal. Int J of Food Sci and Nutri. 2007, 58 (8): 588-594. 10.1080/09637480701343952. 10.1080/09637480701343952View ArticleGoogle Scholar
- Yashodhara BM, Umakanth S, Pappachan JM, Bhat SK, Kamath R, Choo BH: Omega-3 fatty acids, a comprehensive review of their role in health and disease. Postgraduate Med J. 2009, 85: 84-90. 10.1136/pgmj.2008.073338. 10.1136/pgmj.2008.073338View ArticleGoogle Scholar
- Wu J, Johnson LA, Jung S: Demulsification of oil-rich emulsion from enzyme-assisted aqueous extraction of extruded soybean flakes. Bioresource Technol. 2009, 100: 527-533. 10.1016/j.biortech.2008.05.057. 10.1016/j.biortech.2008.05.057View ArticleGoogle Scholar
- Xu CZ, Wang QJ, Zheng MS, Zhu JW, Li JD, Huang MQ, Jia QM, Du ZZ: Microstructure and properties of ultra-fine grain Cu–Cr alloy prepared by equal-channel angular pressing mater. Engineering and Sci. 2007, 459: 303-308. 10.1016/j.msea.2007.01.105. 10.1016/j.msea.2007.01.105View ArticleGoogle Scholar
- Martínez-Flores HE, Barrera ES, Garnica-Romo MG, Penagos CJC, Saavedra JP, Macazaga-Alvarez R: Functional characteristics of protein flaxseed concentrate obtained applying a response surface methodology. J of Food Sc. 2006, 71: 495-498. 10.1111/j.1750-3841.2006.00147.x. 10.1111/j.1750-3841.2006.00147.xView ArticleGoogle Scholar
- Wu M, Li D, Wang L, O’zkan N, Mao ZH: Rheological properties of extruded dispersions of flaxseed-maize blend. J Food Eng. 2010, 98: 480-491. 10.1016/j.jfoodeng.2010.01.031. 10.1016/j.jfoodeng.2010.01.031View ArticleGoogle Scholar
- Betti M, Perez TI, Zuidhof MJ, Renema RA: Omega-3 enriched broiler meat, 3 fatty acid distribution between triacylglycerol and phospholipid classes. Poult Sci. 2009, 88: 1740-1754. 10.3382/ps.2008-00449View ArticlePubMedGoogle Scholar
- Wu M, Li D, Wang L, Zhou Y, Brooks MS, Chen XD: Extrusion detoxification technique on flaxseed by uniform design optimization. Sep Purif Technol. 2008, 61 (1): 51-59. 10.1016/j.seppur.2007.09.016. 10.1016/j.seppur.2007.09.016View ArticleGoogle Scholar
- Minami M, Yoshikawa H: A simplified assay method of superoxide dismutase activity for clinical use. Clinica Chimica Acta. 1979, 92: 337-342. 10.1016/0009-8981(79)90211-0. 10.1016/0009-8981(79)90211-0View ArticleGoogle Scholar
- Aebi H: Catalase estimation. Methods of enzymatic analysis. Edited by: Bergmeyer HU. 1974, 673-684. Verlag chemic, Weinheim, New York.View ArticleGoogle Scholar
- Helen A, Vijayammal PL: Vitamin C supplementation on hepatic oxidative stress induced cigarette smoke. J Appl Toxicol. 1997, 17: 289-295. 10.1002/(SICI)1099-1263(199709)17:5<289::AID-JAT448>3.0.CO;2-9View ArticlePubMedGoogle Scholar
- Beutler E, Duron O, Kelly BM: Improved method for the determination of blood glutathione. J Lab Clin Med. 1983, 61: 882-888.Google Scholar
- Brand-Williams W, Cuvelier ME, Berset C: Use of a free radical method to evaluate antioxidant. Lebensm.- Wissenschaft u.-Techno. 1995, 28: 25-30.View ArticleGoogle Scholar
- Ahn DU, Sell JL, Chen CJX, Wu C, Lee JI: Effects of dietary vitamin E supplementation on lipid oxidation and volatiles content of irradiated, cooked turkey meat patties with different packaging. Poult Sci. 1998, 6: 912-920.View ArticleGoogle Scholar
- Sukhiia P, Palmquist DL: Rapid method for determination of total fatty acid content and composition of feedstuffs and feaces. J of Agri and Food Chem. 1988, 86: 1202-1206.View ArticleGoogle Scholar
- Perlo F, Bonato P, Teira G, Marcuzzi A: Influencia del nu´ Mero de lavados en el procesamiento de carne de ave meca´ nicamente recuperada. Ciencia y Tecnología Alimentaria. 2003, 4 (1): 55-59. 10.1080/11358120309487619. 10.1080/11358120309487619View ArticleGoogle Scholar
- Sallama KI, Ishioroshib M, Samejima K: Antioxidant and antimicrobial effects of garlic in chicken sausage. Lebensm.- Wissenschaft u.-Technol. 2004, 37: 849-855.View ArticleGoogle Scholar
- El-Gasim EA, Al-Wesali MS: Water activity and hunter color values of beef patties extended with samh (mesembryanthemum forsskalei hochst) flour. Food Chem. 2000, 69: 181-185. 10.1016/S0308-8146(99)00252-6. 10.1016/S0308-8146(99)00252-6View ArticleGoogle Scholar
- Cosenza GH, Williams SKD, Johnson D, Sims C, McGowan CH: Development and evaluation of a cabrito smoked sausage product. Meat Sci. 2003, 64: 119-124. 10.1016/S0309-1740(02)00148-1View ArticlePubMedGoogle Scholar
- Carlos ML, Mendes RC, Maria LN: Instrumental texture and sensory characteristics of cod frankfurter sausages. Int J l of Food Propert. 2009, 12: 625-643. 10.1080/10942910801992959. 10.1080/10942910801992959View ArticleGoogle Scholar
- Meilgaard MM, Civille GV, Carr T: Overall difference tests, Does a sensory difference exist between samples? Sensory evaluation techniques. 2007, 63-104. USA, New York, 4.Google Scholar
- Steel RGD, Torrie JH, Dickey D: Principles and procedures of statistics, a biometrical approach. 1997, McGraw Hill Book Co Inc, New York, 3.Google Scholar
- Shen Y, Feng D, Oresanya T, Chavez E: Fatty acid and nitrogen utilization of processed flaxseed by adult chickens. J Sci Food Agri. 2005, 85: 1137-1142. 10.1002/jsfa.2073. 10.1002/jsfa.2073View ArticleGoogle Scholar
- Rodriguez ML, Alzueta C, Rebole A, Ortiz LT, Centeno C, Trevino J: Effect of inclusion level of linseed on the nutrient utilisation of diets for growing broiler chickens. British Poult Sci. 2001, 42: 368-375. 10.1080/00071660120055359. 10.1080/00071660120055359View ArticleGoogle Scholar
- Zuidhof MJ, Betti M, Korver DR, Hernandez FIL, Schneider BL, Carney VL, Renema RA: Omega-3-enriched broiler meat, 1. Optimization of a production system. Poult Sci. 2009, 88: 1108-1120. 10.3382/ps.2008-00171View ArticlePubMedGoogle Scholar
- Nguyen CV, Smulikowska S, Mieczkowska A: Effect of linseed and rapeseed or linseed and rapeseed oil on performance, slaughter yield and fatty acid deposition in edible parts of the carcass in broiler chickens. J Anim Feed Sci. 2003, 12: 271-288.Google Scholar
- Maraschiello C, SaÂ´ rraga C, Garcia Regueiro JA: Glutathione peroxidase activity, TBARS, and α-tocopherol in meat from chickens Fed different diets. J Agri Food Chem. 1999, 1999 (47): 867-872.View ArticleGoogle Scholar
- Rodriguez-Martinez MA, Ruiz-Torres A: Homeostasis between lipid peroxidation and antioxidant enzyme activities in healthy human aging. Mech Ageing Dev. 2001, 66: 213-222.View ArticleGoogle Scholar
- Luna A, Labaque MC, Zygadlo JA, Marin RH: Effects of thymol and carvacrol feed supplementation on lipid oxidation in broiler meat. Poult Sci. 2010, 89: 366-370. 10.3382/ps.2009-00130View ArticlePubMedGoogle Scholar
- Zanini SF, Colnago GL, Bastos MR, Pessotti BMS, Casagrande FP, Lima VR: Oxidative stability and total lipids on thigh and breast meat of broilers fed diets with two fat sources and supplemented with conjugated linoleic acid. LWT- Food Sci Technol. 2006, 39: 717-723. 10.1016/j.lwt.2005.05.005. 10.1016/j.lwt.2005.05.005View ArticleGoogle Scholar
- Bradley PA, Edel AL, McCullough R, Rodriguez-Leyva D, Rampersad P, Gilchrist JSC, Lukas A, Pierce GN: Distribution of omega-3 fatty acids in tissues of rabbits fed a flaxseed-supplemented diet. Metab Clin Exp. 2010, 2010 (59): 620-627.Google Scholar
- Juarez M, Dugan MER, Aldai N, Aalhus JL, Patience JF, Zijlstra RT, Beaulieu AD: Increasing omega-3 levels through dietary co-extruded flaxseed supplementation negatively affects pork palatability. Food Chem. 2010, 126: 1716-1723.View ArticlePubMedGoogle Scholar
- Jeun-Hornga L, Yuan-Huib L, Chun-Chinc K: Effect of dietary fish oil on fatty acid composition, lipid oxidation and sensory property of chicken frankfurters during storage. Meat Sci. 2002, 60: 161-167. 10.1016/S0309-1740(01)00118-8View ArticleGoogle Scholar
- CaÂ´ ceres E, Garcia ML, Selgas MD: Effect of pre-emulsified fish oil as source of PUFA n-3 on microstructure and sensory properties of mortadella, a Spanish bologna-type sausage. Meat Sci. 2008, 80: 183-193. 10.1016/j.meatsci.2007.11.018View ArticleGoogle Scholar
- Hoz L, D’Arrigo M, Cambero I, Ordonez JA: Development of an n-3 fatty acid and a-tocopherol enriched dry fermented sausage. Meat Sci. 2004, 67 (3): 485-495. 10.1016/j.meatsci.2003.12.001View ArticlePubMedGoogle Scholar
- Sohaib M, Anjum FM, Khan MI, Arshad MS, Shahid M: Enhancement of lipid stability of broiler breast meat and meat products fed on alpha lipoic acid and alpha tocopherol acetate supplemented feed. J Lipids health Dis. 2012, 11: 57-10.1186/1476-511X-11-57. 10.1186/1476-511X-11-57View ArticleGoogle Scholar
- Barbut S: Poultry products processing, an industry guide. 2002, CRC Press, Boca Raton, Florida.Google Scholar
- Woods VB, Fearon EA: Dietary sources of unsaturated fatty acids for animals and their transfer into meat, milk and eggs, a review. Livest Sci. 2009, 126: 1-20. 10.1016/j.livsci.2009.07.002. 10.1016/j.livsci.2009.07.002View ArticleGoogle Scholar
- Valencia I, Ansorena D, Astiasaran I: Nutritional and sensory properties of dry fermented sausages enriched with n-3 PUFAs. Meat Sci. 2006, 72 (4): 727-733. 10.1016/j.meatsci.2005.09.022View ArticlePubMedGoogle Scholar
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