Effects of butter naturally enriched with conjugated linoleic acid and vaccenic acid on blood lipids and LDL particle size in growing pigs
© Haug et al; licensee BioMed Central Ltd. 2008
Received: 27 June 2008
Accepted: 29 August 2008
Published: 29 August 2008
Cow milk is a natural source of the cis 9, trans 11 isomer of conjugated linoleic acid (c9,t11-CLA) and trans vaccenic acid (VA). These fatty acids may be considered as functional foods, and the concentration in milk can be increased by e.g. sunflower oil supplementation to the dairy cow feed.
The objective of this study was to compare the effects of regular butter with a special butter naturally enriched in c9,t11-CLA and VA on plasma lipids in female growing pigs. The experimental period lasted for three weeks and the two diets provided daily either 5.0 g c9,t11-CLA plus 15.1 g VA or 1.3 g c9,t11-CLA plus 3.6 g VA.
The serum concentrations of c9,t11-CLA, VA and alpha-linolenic acid were increased and myristic (14:0) and palmitic acid (16:0) were reduced in the pigs fed the CLA+VA-rich butter-diet compared to regular butter, but no differences in plasma concentrations of triacylglycerol, cholesterol, HDL-cholesterol, LDL-cholesterol, LDL particle size distribution or total cholesterol/HDL cholesterol were observed among the two dietary treatment groups.
Growing pigs fed diets containing butter naturally enriched in about 20 g c9,t11-CLA plus VA daily for three weeks, had increased serum concentrations of alpha-linolenic acid and decreased myristic and palmitic acid compared to pigs fed regular butter, implying a potential benefit of the CLA+VA butter on serum fatty acid composition. Butter enriched in CLA+VA does not appear to have significant effect on the plasma lipoprotein profile in pigs.
Milk and dairy products have long traditions in human nutrition, but for some decades milk fat has been associated with negative health effects. However, the association between milk fat and plasma lipids is ambiguous, and a paradox. Several studies show no convincing evidence that dairy products increase the risk of cardiovascular disease and that milk is harmful [1–4]. Some studies indicate that a moderate intake of milk fat may reduce the risk of cardiac diseases, possibly through reduced formation of small dense low density lipoprotein particles (sdLDL) . The sdLDL are thought to undergo oxidation more readily, or to be harder bound to the arterial endothelia surface . Dairy milk fat contains numerous fatty acids that might affect formation of sdLDL, such as saturated fatty acids, c9,t11-CLA isomer and VA [7, 8]. Evidence for hypolipidemic properties of c9,t11-CLA has been given, and administration of CLA has been shown to modulate plasma lipid concentration in both human and animal models, and to reduce markers associated with atherogenic risk [8–10]. These findings have led to considerable interest in methods for naturally increasing the c9,t11-CLA content in milk, and milk products that are naturally enriched in CLA has been advocated. CLA is a group of polyunsaturated fatty acids found naturally in beef, lamb and dairy products, and c9,t11-CLA is the main form of CLA (18:2 c9,t11). It can be produced in ruminants by bacterial isomerisation of linoleic acid (18:2 c9,c12) in the rumen. In ruminants and non-ruminants it can be produced in most tissues by delta-9 desaturation of VA (18:1 t11) [11, 12]. The concentration of c9,t11-CLA and VA in milk fat is highly dependent on the feed composition; VA is an intermediate product of biohydrogenation of unsaturated fatty acids in the rumen, and feedstuff rich in linoleic or α-linolenic acids enhance CLA and VA in milk . Since c9,t11-CLA in milk fat is associated with VA, milk rich in c9,t11-CLA is also rich in VA, its precursor . In general, trans fatty acids are associated with increased plasma cholesterol and risk for coronary heart disease. Therefore the concentration of VA in milk has been of concern. However, VA's effect on plasma cholesterol has not been entirely understood since epidemiological studies have shown that trans fatty acids from animal sources did not increase risk for coronary artery disease .
The objective of the study presented here was to compare effects on plasma lipid- and fatty acid profile in growing pigs that had been given diets containing regular butter (REG) or butter naturally enriched in CLA plus VA.
Results and discussion
Feed intake and weight gain
In the present study, there were no significant differences in the feed intake (in average 1.65 kg and 1.67 kg per day), weight gain (17.9 kg and 18.4 kg) and final body weight (61.2 kg and 61.9 kg) in the REG and CLA+VA treatment groups, respectively (data not shown). The CLA isomer involved in decreasing body fat is the t10,c12-CLA isomer, but there are no concluding evidence of such effects of the c9,t11-CLA isomer [15, 16].
Fatty acid (FA) composition of diets
Fatty acid composition of the diets, (g/100 g fatty acid methyl ester).
To provide a high intake of natural CLA and VA, the experimental diets were rich in fat; 19% fat in both diets, giving as much as 46% of the energy (E%) from fat. The pigs liked the diets, and in accordance to others  pigs tolerated well a high fat diet.
Serum fatty acids
Overall means in serum fatty acid concentration (g/100 g fatty acid methyl ester)a
0.17 ± 0.05
0.12 ± 0.03
0.67 ± 0.44
0.54 ± 0.13
1.36 ± 0.31
1.03 ± 0.12
0.32 ± 0.04
0.28 ± 0.05
20.1 ± 0.90
17.1 ± 0.75
1.02 ± 0.17
0.89 ± 0.13
16.3 ± 1.6
14.6 ± 0.9
16.2 ± 1.7
16.2 ± 0.3
20.0 ± 1.3
21.6 ± 2.4
0.81 ± 0.13
1.02 ± 0.09
0.27 ± 0.05
1.09 ± 0.18
0.28 ± 0.08
0.80 ± 0.07
8.2 ± 1.0
8.1 ± 0.4
1.49 ± 0.19
1.46 ± 0.06
1.41 ± 0.15
1.47 ± 0.19
1.74 ± 0.34
1.46 ± 0.17
The main FA in serum are palmitic acid, stearic acid, oleic acid and linoleic acid, together accounting for about 70% of total FA (Table 2). Pigs fed the CLA+VA diet had a 3.4 fold higher serum concentrations of c9,t11-CLA plus VA compared to the REG diet group. Alpha-linolenic acid was 25% higher, and myristic and palmitic acid was 25% and 15% lower in the serum of CLA+VA compared to the REG dietary treatment (Table 2). The mirroring effect of dietary FA on serum FA is in accordance to several studies [5, 18, 19]. The favourable increased serum concentrations of the omega-3 fatty acid α-linolenic acid and the decrease in the saturated palmitic and myristic acids may indicate that the CLA+VA rich butter can have a positive role in the western diet.
Plasma cholesterol, triacylglycerol and lipoproteins
At the start of the study, no differences between the groups were observed for plasma concentrations of total cholesterol, LDL-cholesterol, high density lipoprotein (HDL)-cholesterol, sdLDL subclass particle diameter, the ratio between total cholesterol and HDL cholesterol, free fatty acids and triacylglycerol.
Plasma lipids, lipoproteins and percent distribution of LDL-particles LDL-I, LDL-II, LDL-III and LDL-IVa
0.43 ± 0.07
0.46 ± 0.13
Total cholesterol mmol/l
3.61 ± 0.5
3.50 ± 0.4
HDL cholesterol mmol/l
1.44 ± 0.17
1.32 ± 0.21
LDL cholesterol (calculated)
1.98 ± 0.37
1.97 ± 0.33
Total cholesterol/HDL cholesterol
2.51 ± 0.15
2.65 ± 0.28
LDL cholesterol/HDL cholesterol
1.38 ± 0.15
1.49 ± 0.28
LDL peak size (Å)
243 ± 3.6
243 ± 3.2
21.7 ± 7.1
25.0 ± 4.0
47.5 ± 3.4
44.9 ± 1.9
20.5 ± 4.2
18.5 ± 3.3
8.0 ± 1.8
9.2 ± 1.7
Nonesterified fatty acids mmol/l
0.48 ± 0.18
0.41 ± 0.09
Milk products have been shown to have an apparently beneficial effect on LDL particle size distribution (giving less of the sdLDL) . From the Framingham Offspring Study  it has been shown that subjects with a high intake of certain saturated fatty acids (4:0–10:0 and myristic acid) abundantly found in milk products, have lowered levels of sdLDL. Other has shown that saturated fatty acids (especially myristic- and palmitic acid) may affect the distribution of the LDL particles, giving more of the large LDLs . The REG diet contained more 10:0, myrisitic and palmitic acid, but no improvement in LDL particle size was observed in the REG diet group.
A diet containing natural CLA+VA enriched butter resulted in increased serum concentrations of CLA, vaccenic acid and α-linolenic acid, and reduced concentration of myristic and palmitic acid in pigs compared to a diet containing regular butter, indicating a potential health benefit of the CLA+VA rich butter. However, no differences in plasma lipoproteins and LDL particle sizes were observed among the two dietary treatment groups following three weeks feeding. It is worth noting that a relatively high intake of the trans fatty acid VA did not result in a detrimental effect on the lipoprotein profile when it was in combination with c9,t11-CLA. Perhaps is the combination of fatty acids in milk fat one reason to the milk fat paradox?
The experimental research on animals followed internationally recognized guidelines. All animals were cared for according to laws and regulations controlling experiments with live animals in Norway (The Animal Protection Act of December 20th, 1974, and the Animal Protection Ordinance Concerning Experiments with Animals of January 15th, 1996); according to the rules given by Norwegian Animal Research Authority.
Animals and diets
Twelve growing female pigs (initial weight were 43.4 ± 1.5 kg) of a commercial Norwegian crossbreed ((Landrace × Yorkshire) × (Landrace × Duroc)) were selected for the study. The pigs were reared indoors, and fed two times per day in accordance to NRC requirements for nutrients for growing pigs . A veterinarian examined the pigs every week.
Composition of experimental diets, (g per 100 g dry feed).
CLA rich butter-fat
The experimental period lasted for three weeks. Twelve pigs were randomized into two groups (n = 6) and individually fed one of two diets; CLA+VA or REG. The pigs were weighed once every week and amount of feed was adjusted according to body weight. At feeding time, pigs were restrained in an individual feeding stall for about 1/2 h, and feed intake was recorded.
Blood samples were obtained by vena cava puncture at the start and at the end of the experiment. Blood samples were taken after an overnight fasting in Na-heparin-, EDTA- and empty vacuum tubes. Blood samples were immediately chilled on ice. Plasma and serum were obtained by low speed centrifugation for 20 min at 1700 g. Plasma, serum and whole blood (heparin blood) were frozen and kept at -80°C until analyzed.
Fatty acid analyses
Fatty acid composition in serum, feed concentrate, palm oil and butter were determined by gas chromatography. Lipids were extracted according to Folch et al. . For lipid extraction from serum a modified method was used: 0.2 ml serum was mixed with 0.3 ml 0.5 M KH2 PO4, 1.5 ml chloroform and 0.5 ml methanol. After centrifugation at 1700 g for 10 minutes, the lower phase was transferred to new tubes, the solvents were evaporated by N2, and lipids resolved in heptane. Fat from serum and diet were methylated by the method described by Kramer et al. , using both sodium methoxide and methanolic HCl 3N (Supelco, PA, USA). Subsequently, the fatty acid methyl esters were analyzed using a Finnigan Focus gas chromatograph with a 100 m capillary column (CP Sil 88 WCOT, 100-m × 0.25 mm, Chrompack, Middelburg, Netherland). Peak areas of fatty acids were used to calculate the amount of fatty acids (g/100 g fat) by theoretical response factors . Standard fatty acids of known composition were run to identify the fatty acids in the samples. Plasma control samples were extracted, methylated and analysed by every 10th sample.
The heparin-plasma analyses were carried out on a Cobas Mira autoanalyzer using the following kits: nonesterified fatty acids (NEFA) (NEFA C ACS-ACOD method. Wako Chemicals, VA, USA), triacylglycerol (Triglycerides 100, ABX diagnostics, Montpellier, France), total cholesterol (Cholesterol 100–250, ABX diagnostics, Montpellier, France), high density lipoprotein-cholesterol (HDL-cholesterol direct, ABX diagnostics, Montpellier, France) and glucose (Glucose HK 125 kit, ABX diagnostics, Montpellier, France). Low density lipoptotein-(LDL) cholesterol was calculated using the Friedewald equation . The interassay coefficients of variation were the following: total cholesterol 2%, HDL-cholesterol 5%, triacylglycerols 3%, NEFA 2.5%.
LDL particle size distribution was determined by gradient gel electrophoresis as described by Sjogren et al. . Briefly, a lipoprotein-rich fraction (containing very low density lipoprotein (VLDL) to LDL) was isolated from freshly thawed EDTA-plasma by adjusting the density to 1.070 kg/L and subsequent ultracentrifugation (142500 g for 22 h, 4°C). Recovery of total plasma apoB was 77 ± 12% (n = 8). The lipoprotein-rich fraction was applied to a 3–7.5% polyacrylamide gel together with standard lipoproteins (isolated human Lp(a) and LDL) and proteins (thyroglobulin mono- and dimer, Pharmacia, LKB, Stockholm, Sweden) of known size and run for 20 h at 80 V. Gels were stained for protein (0.04% Coomassie Brilliant Blue, Serva, Heidelberg, Germany) and analyzed using a Fuji LAS-1000 system and Image Gauge software to give peak particle size of LDL and relative distribution of LDL in predefined subfractions with cut-offs: LDL-I (27.0–25.0 nm), LDL-II (25.0–23.5 nm), LDL-III (23.5–22.5 nm) and LDL-IV (22.5–21.0 nm), corresponding to densities of 1.006–1.030, 1.030–1.040, 1.040–1.050 and 1.050–1.063 kg/L, respectively. A density of 1.040 kg/L is a classic boundary for dividing LDL into large and small particles  rendering LDL subclasses III and IV as small dense LDL with this method.
The results of the plasma and serum analyses are presented as mean values, and standard deviation and p-values are given. Data were analyzed by using the statistical package in Microsoft Office Excel, 2003, using TTEST, two-tailed distribution and two-sample equal variance.
We are grateful to Tine BA, Oslo, Norway for support and providing the butter and funds for the laboratory analyses in this study, several researchers at the Norwegian University of Life Sciences for spending time working with the study and writing the manuscript, personnel working at the animal unit (SHF) for conducting the feeding experiments, staff at the Norwegian School of Veterinary Science, Oslo, Norway for taking blood samples, the staff working at the laboratories at the Norwegian University of Life Sciences for blood analyses and the research staff at Karolinske Institutet, Sweden for providing the LDL particle size analyses.
The sources of funding in study design and practical work, manuscript preparation and interpretation of data for each author in the study: AH, NH, NPK, HM, OT, OMH: Norwegian University of Life Sciences, Aas, Norway, PS: Karolinska Institutet, Stockholm, Sweden, ASB and ESO; Tine AB, Norway.
- Sandstrom B, Marckmann P, Bindslev N: An eight-month controlled study of a low-fat high-fibre diet: effects on blood lipids and blood pressure in healthy young subjects. Eur J Clin Nutr. 1992, 46: 95-109.PubMedGoogle Scholar
- Elwood PC, Pickering JE, Hughes J, Fehily AM, Ness AR: Milk drinking, ischaemic heart disease and ischaemic stroke II. Evidence from cohort studies. Eur J Clin Nutr. 2004, 58: 718-724. 10.1038/sj.ejcn.1601869View ArticlePubMedGoogle Scholar
- Seidel C, Deufel T, Jahreis G: Effects of fat-modified dairy products on blood lipids in humans in comparison with other fats. Ann Nutr Metab. 2005, 49: 42-48. 10.1159/000084176View ArticlePubMedGoogle Scholar
- Tholstrup T, Hoy CE, Andersen LN, Christensen RD, Sandstrom B: Does fat in milk, butter and cheese affect blood lipids and cholesterol differently?. J Am Coll Nutr. 2004, 23: 169-176.View ArticlePubMedGoogle Scholar
- Sjogren P, Rosell M, Skoglund-Andersen M, Zdravkovic S, Vessby B, deFaire U, Hamsten A, Hellenius ML, Fisher R: Milk-derived fatty acids are associated with a more favorable LDL particle size distribution in healthy men. J Nutr. 2004, 134: 1729-35.PubMedGoogle Scholar
- de Graaf J, Hak-Lemmers HL, Hectors MP, Demacker PN, Hendriks JC, Stalenhoef AF: Enhanced susceptibility to in vitro oxidation of the dense low density lipoprotein subfraction in healthy subjects. Arterioscler Thromb. 1991, 11: 298-306.View ArticlePubMedGoogle Scholar
- Kramer JK, Cruz-Hernandez C, Deng Z, Zhou J, Jahreis G, Dugan ME: Analysis of conjugated linoleic acid and trans 18:1 isomers in synthetic and animal products. Am J Clin Nutr. 2004, 79: 1137S-1145S.PubMedGoogle Scholar
- Tricon S, Burdge GC, Kew S, Banerjee T, Russell JJ, Jones EL, Grimble RF, Williams CM, Yaqoob P, Calder PC: Opposing effects of cis-9, trans-11 and trans-10, cis-12 conjugated linoleic acid on blood lipids in healthy humans. Am J Clin Nutr. 2004, 80: 614-20.PubMedGoogle Scholar
- Tricon S, Burdge GC, Jones EL: Effects of dairy products naturally enriched with cis-9, trans-11 conjugated linoleic acid on the blood lipid profile in healthy middle-aged men. Am J Clin Nutr. 2006, 83: 744-53.PubMedGoogle Scholar
- Valeille K, Gripois D, Blouquit MF, Souidi M, Riottot M, Bouthegourd JC, Serougne C, Martin JC: Lipid atherogenic risk markers can be more favourably influenced by the cis-9, trans-11-octadecadienoate isomer than a conjugated linoleic acid mixture or fish oil in hamsters. Br J Nutr. 2004, 91: 191-9. 10.1079/BJN20031057View ArticlePubMedGoogle Scholar
- Turpeinen AM, Mutanen M, Aro A, Salminen I, Basu S, Palmquist DL, Griinari JM: Bioconversion of vaccenic acid to conjugated linoleic acid in humans. Am J Clin Nutr. 2002, 76: 504-10.PubMedGoogle Scholar
- Jenkins TC, Wallace RJ, Moate PJ, Mosley EE: Board-invited review: Recent advances in biohydrogenation of unsaturated fatty acids within the rumen microbial ecosystem. J Anim Sci. 2008, 86: 397-412. 10.2527/jas.2007-0588View ArticlePubMedGoogle Scholar
- Bu DP, Wang JQ, Dhiman TR, Liu SJ: Effectiveness of oils rich in linoleic and linolenic acids to enhance conjugated linoleic acid in milk from dairy cows. J Dairy Sci. 2007, 90: 998-1007.View ArticlePubMedGoogle Scholar
- Willett WC, Stampfer MJ, Manson JE: Intake of trans fatty acids and risk of coronary heart disease among women. Lancet. 1993, 341: 581-5. 10.1016/0140-6736(93)90350-PView ArticlePubMedGoogle Scholar
- Park Y, Albright KJ, Storkson JM, Liu W, Cook ME, Pariza MW: Changes in body composition in mice during feeding and withdrawal of conjugated linoleic acid. Lipids. 1999, 34: 243-8. 10.1007/s11745-999-0359-7View ArticlePubMedGoogle Scholar
- Dugan ME, Aalhus JL, Kramer JK: Conjugated linoleic acid pork research. Am J Clin Nutr. 2004, 79 (6 Suppl): 1212S-1216S.PubMedGoogle Scholar
- Leibbrandt VD, Ewan RC, Speer VC, Zimmerman DR: Effect of age and calorie: Protein ratio on performance and body composition of baby pigs. J Animal Sci. 1975, 40: 1070-76.Google Scholar
- Smith DR, Knabe DA, Cross HR, Smith SB: A diet containing myrisitoleic plus palmitoleic acids elevates plasma cholesterol in young growing swine. Lipids. 1996, 31: 849-58. 10.1007/BF02522980View ArticlePubMedGoogle Scholar
- Smedman AE, Gustafsson IB, Berglund LG, Vessby BO: Pentadecanoic acid in serum as a marker for intake of milk fat: relations between intake of milk fat and metabolic risk factors. Am J Clin Nutr. 1999, 69: 22-9.PubMedGoogle Scholar
- Wolfram G: Dietary fatty acids and coronary heart disease. Eur J Med Res. 2003, 8: 321-4.PubMedGoogle Scholar
- Luhman CM, Faidley TD, Beitz DC: Postprandial lipoprotein composition in pigs fed diets differing in type and amount of dietary fat. J Nutr. 1992, 122: 120-7.PubMedGoogle Scholar
- Ascherio A: Trans fatty acids and blood lipids. Atheroscler Suppl. 2006, 7: 25-7. 10.1016/j.atherosclerosissup.2006.04.018View ArticlePubMedGoogle Scholar
- Campos H, Blijlevens E, McNamara JR, Ordovas JM, Posner BM, Wilson PW, Castelli WP, Schaefer EJ: LDL particle size distribution. Results from the Framingham Offspring Study. Arterioscler Thromb. 1992, 12: 1410-9.View ArticlePubMedGoogle Scholar
- Dreon DM, Fernstrom HA, Campos H, Blanche P, Williams PT, Krauss RM: Change in dietary saturated fat intake is correlated with change in mass of large low-density-lipoprotein particles in men. Am J Clin Nutr. 1998, 67: 828-36.PubMedGoogle Scholar
- NRC: Nutrient requirements of swine. 1988, National Academy Press, Washington, D.C, 9,Google Scholar
- Folch J, Lees M, Stanley GHS: A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem. 1957, 226: 497-509.PubMedGoogle Scholar
- Kramer JKG, Fellner V, Dugan MER, Sauer FD, Mossoba MM, Yurawecz MP: Evaluating acid and base catalyst in the methylation of milk and rumen fatty acids with special emphasis on conjugated dienes and total trans fatty acids. Lipids. 1997, 32: 1219-28. 10.1007/s11745-997-0156-3View ArticlePubMedGoogle Scholar
- Ackman RG, Sipos JC: Flame ionization detector response for the carbonyl carbon atom in the carboxyl group of fatty acids and esters. J Chromatogr. 1964, 16: 298-305. 10.1016/S0021-9673(01)82491-2View ArticlePubMedGoogle Scholar
- Friedewald WT, Levy RI, Fredrickson DS: Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem. 1972, 18: 499-502.PubMedGoogle Scholar
- Skoglund-Andersson C, Tang R, Bond MG, deFaire U, Hamsten A, Karpe F: LDL particle size distribution is associated with carotid intima-media thickness in helathy 50-year-old men. Arterioscler Thromb Vasc Biol. 1999, 19 (10): 2422-30.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.