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Common variants in the genes of triglyceride and HDL-C metabolism lack association with coronary artery disease in the Pakistani subjects

Lipids in Health and Disease volume 16, Article number: 24 (2017) Cite this article

Abstract

Background

Serum Triglyceride (TG) and High Density Lipoprotein (HDL-C) levels are modifiable coronary artery disease (CAD) risk factors. Polymorphisms in the genes regulating TG and HDL-C levels contribute to the development of CAD. The objective of the current study was to investigate the effect of four such single nucleotide polymorphism (SNPs) in the genes for Lipoprotein Lipase (LPL) (rs328, rs1801177), Apolipoprotein A5 (APOA5) (rs66279) and Cholesteryl ester transfer protein (CETP) (rs708272) on HDL-C and TG levels and to examine the association of these SNPs with CAD risk.

Methods

A total of 640 subjects (415 cases, 225 controls) were enrolled in the study. The SNPs were genotyped by KASPar allelic discrimination technique. Serum HDL-C and TG were determined by spectrophotometric methods.

Results

The population under study was in Hardy Weinberg equilibrium and minor allele of SNP rs1801177 was completely absent in the studied subjects. The SNPs were association with TG and HDL-C levels was checked through regression analysis. For rs328, the effect size of each risk allele on TG and HDL-C (mmol/l) was 0.16(0.08) and −0.11(0.05) respectively. Similarly, the effect size of rs662799 for TG and HDL-C was 0.12(0.06) and −0.13(0.0.3) and that of rs708272 was 0.08(0.04) and 0.1(0.03) respectively. The risk allele frequencies of the SNPs were higher in cases than controls, but the difference was not significant (p > 0.05) and SNPs were not associated with CAD risk (p > 0.05). The combined gene score of four SNPs significantly raised TG and lowered HDL-C but did not increase CAD risk.

Conclusion

The studied SNPs were associated with TG and HDL-C levels, but not with CAD in Pakistani population under study.

Background

Lipoprotein lipase (LPL), a 61 kDa heparin releasable enzyme is attached to capillary endothelium at glycosaminoglycan residues [1–3]. It is a rate limiting enzyme in hydrolysis of triglycerides (TG) from core of chylomicrons (CM) and very low density lipoproteins (VLDL) into free fatty acids and glycerol generating CM remnants from CM, intermediate density lipoprotein (IDL) from VLDL and eventually low density lipoprotein (LDL) cholesterol by the action of hepatic lipase. Phospholipids and surface free cholesterol are converted to HDL-C [4]. Decreased LPL activity is associated with increased TG and decreased HDL-C levels and accumulation of atherogenic TG rich lipoproteins in circulation [5–8]. Although affected by environmental factors and life style, genetic variants within a population may also influence TG and HDL-C levels. It has been estimated that heritability accounts for >30% hypertriglyceridemia cases [9].

The LPL gene is highly polymorphic and more than 100 variants have been identified in it [10]. The common variants with modest effect on TG and HDL-C levels may prove to be an important CAD risk contributors at population levels. The polymorphism LPL rs328 is a C > G change in exon nine and changes the codon for serine 447 (TCA) to TGA which is a stop codon hence also given the name Ser447Stop or S447X. This results in pre-mature truncation of the protein which is short of two amino acids. The product of LPLS447X is missing C terminal serine and glycine residues and also generates restriction site for the enzyme MnlI [11]. LPL rs1801177 is a mis-sense polymorphism and causes substitution of aspartic acid (D) to asparagine (N) hence also denoted as Asp9Asn or D9N.

Apo-lipoprotein A5 (ApoA5) is part of VLDL, HDL and CM and is a major regulator of blood TG and HDL-C through interaction with LDLR [12]. ApoA5 controls TG levels directly by increasing the breakdown of TG rich lipoproteins through the action of LPL and indirectly by reducing VLDL biosynthesis [13]. The expression of ApoA5 is in inverse relation with circulating plasma TG levels. ApoA5 knockout mice had 4 folds higher TG concentration and when knocked in with human ApoA5, it caused a 50-70% reduction in mouse serum TG level [14]. The APOA5 rs662799 is the most studied SNP in the promoter region of this gene [15]. Different allele frequencies of this polymorphism have been reported in Chinese and Europeans [16] and is the most effective SNP in ApoA5 gene [17]. The SNP is associated with decreased ApoA5 levels and high plasma TG levels and the association has been shown to be independent of other factors.

Cholesteryl ester transfer protein (CETP) transfers lipids between lipoproteins and redistributes triglycerides, cholesteryl esters and phospholipids between lipoproteins [18, 19]. An inverse correlation exists between CETP activity and blood HDL-C levels [20]. Many functional polymorphisms have been identified in CETP gene [21]. The association of CETP with HDL-C is stronger than any other locus in the genome [22]. The polymorphism CETP rs708272 also known as Taq1B polymorphism in this gene is the most studied one. The effect of this SNP is also modulated by the factors like smoking and alcohol consumption [21].

The effect of these common variants in the genes for lipid metabolism on blood lipid levels has been studied well in Caucasians. However, the data on Pakistani people is limited. As is known that the allele frequencies and linkage disequilibrium of a SNP with tag/functional SNP may vary among ethnicities, the authors of the study hypothesized that the SNPs in the above mentioned genes may have strong effect on blood TG and HDL-C levels in Pakistani population which is facing high CAD burden. In this study, we examined the effect of polymorphisms LPL rs328, LPL rs1801177, ApoA5 rs662799 and CETP rs708272 on serum TG and HDL-C levels and their association with CAD. For this purpose, a case control study was opted. As the common variants individually have modest effects, the cumulative effect of the four SNPs was examined by combining them in a small gene score.

Methods

Study subjects

A total of 640 subjects including 415 CAD cases and 225 healthy controls were enrolled in the study. The inclusion criteria for selection of cases and controls has been already described [23]. The CAD patients were selected from tertiary care hospitals of the province of Punjab, Pakistan. These were diagnosed cases based upon the ECG, cardiac echo, radiologic and troponine T/I levels. These cases were recently diagnosed of having CAD and were not taking any lipid lowering or antihypertensive drug. Control subjects were apparently healthy individuals from the general population with BMI 18.5-22.99 Kg/m2 and no history of heart disease. The exclusion criteria included the presence of any chronic ailment like chronic liver or kidney disease, cancers or any ongoing acute infection or disease and CAD patients with obesity. The participants gave a written informed consent and the study was approved by the institutional ethical committee, university of the Punjab, Lahore.

Biochemical and genetic analysis

Serum TG and HDL-C were measured using commercially available kits (Human diagnostics, Germany). A 96 well plate reader, Epoch (Biotek instruments, Highland Park) was used for end stage spectrophotometric measurements. The DNA was extracted from whole blood leucocytes using commercially available genomic DNA purification kit (Promega Wizard, USA). The DNA was quantified using nano drop (ND-8000, USA). The DNA samples were arrayed into 384 well plates (Micro Amp) by an automated robotic liquid handling system (Biomerk FX, Beckman Coulter). The SNPs were genotyped by florescence based allelic discrimination technique KASPar, the details described elsewhere [24]. After amplification, the results were analysed on ABI Prism 7900HT (Applied Biosystems/Life Technologies) and the genotypes were called by sequence detection software (SDS, version 2.0). The sequence of primers and probes used for KASPar are given in Additional file 1: Table S1. The genotyping results were also confirmed by direct DNA sequencing (Source Biosciences, UK) and the results were always same. The DNA fragment containing polymorphism was amplified using commercially available master mix (Qiagen). The sequence of primers used for direct DNA sequencing is given in Additional file 2: Table S2. The genotyping quality was also checked by the inclusion of non-template controls and known heterozygotes in the test plate.

Statistical analysis

The results were analysed using statistical package for social sciences (SPSS), IBM version 22.0. Independent sample t test was used to compare continuous variables between cases and controls. Hardy-Weinberg equilibrium of genotypes in studied subjects was assessed by a chi squared test. The allele frequencies were compared between cases and controls by chi squared test [25]. The association of SNPs with TG and HDL-C was estimated by linear regression. As CAD is a binary variable, the association of SNPs with CAD was calculated by binary logistic regression. One way analysis of variance (ANOVA) was used to calculate mean TG and HDL-C levels along different genotypes. The effect size (β effect) which is per risk allele increase/decrease in TG and HDL-C levels was calculated by linear regression and indicated by β with standard error (Se). Differences in regression slope between groups were tested by fitting an interaction term in the regression model. The relationship between gene score, frequency of individuals with a particular gene score and TG and HDL-C was plotted using an excel spread sheet. For all measurements, statistically significant cut-off was adjusted at a p-value <0.05. An un-weighted gene score of four SNPs was calculated by summing up the number of risk alleles at all the four loci.

Results

The baseline biochemical and anthropometric parameters of the subjects under study are given in Table 1. Serum TG and HDL-C were significantly higher in the cases than the controls. The CAD cases were more hypertensive, diabetic, and smoking rate was also significantly higher than controls. The genotyping rate for all the SNPs was >95%. The basic features of the study SNPs and the Hardy Weinberg equilibrium p-values are provided in the Additional file 3: Table S3. The study population was in Hardy Weinberg equilibrium collectively as well as individually among cases and controls except LPL rs1801177, for which a quite monomorphic picture was observed and risk allele was absent in the subjects studied. In the case of rs328, rs662799 and rs708272, the RAFs were higher in the cases than the controls, but the difference was not statistically significant as the chi squared p values were >0.05. A mean gene score of four SNPs was also not significantly higher in the cases than the controls (Table 2). The SNPs under study were not associated with CAD outcome. The odds ratio (OR) of all the SNPs was higher in the cases than the controls, but was not statistically significant. The combined gene score of 4 SNPs was also not associated with CAD (Table 3).

Table 1 Anthropometric and biochemical features of the subjects under study
Full size table
Table 2 Comparison of RAFs between cases and controls
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Table 3 Association of the SNPs and gene score with CAD
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Association of the selected SNPs with serum triglyceride and high density lipoprotein cholesterol levels

The effect of SNPs on TG and HDL-C levels was examined individually. The risk alleles of all the SNPs raised TG levels, but the β effect was only marginally significant. The risk alleles significantly lowered HDL-C levels in the subjects under study as shown by their β effect p values <0.05 (Table 4). Individually, each risk allele increased TG and decreased HDL-C and the combined gene score was significantly associated with both TG and HDL-C levels (Table 5).

Table 4 Mean TG and HDL-C levels according to genotypes
Full size table
Table 5 Correlation of gene score with TG and HDL-C
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Quantitative effect of SNPs on the serum parameters

The mean increase in TG and a decrease in HDL-C with the increasing number of risk alleles and the difference in TG and HDL-C levels between those having lowest and highest number of risk alleles was measured. It was evident that TG was significantly higher and HDL-C was significantly lower in subjects having highest number of risk allele than those having lowest number of risk alleles (Table 6). The correlation between TG/HDL-C, gene score and CAD was also examined. It was clear that more CAD cases showed skewed distribution towards the high gene score and TG levels. Whereas, more controls were shifted towards the left side of the normal distribution curve with lower gene score and TG levels. Contrarily to TG levels, as low HDL-C is a CAD risk factor, a reverse pattern was observed. The control subjects having higher HDL-C were concentrated towards the left side, whereas more cases were located towards the right side of the graph where the gene score was high (Figs. 1 and 2).

Table 6 The effect of increasing risk allele on TG and HDL-C
Full size table
Fig. 1
figure 1

Green bars represent cases and grey bars represent controls

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Fig. 2
figure 2

Green bars represent cases and grey bars represent controls

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Discussion

The prevalence of coronary artery disease has increased globally, however, the larger proportion of the burden is shared by the developing countries due to larger populations. As a complex disorder, it is difficult to dissect the genetic and environmental components involved in the progression of the disease. The unique social and cultural practices prevailing in the Pakistani population tend to concentrate the risk alleles predisposing to the heart diseases. A large number of genes have been identified to be associated with the risk of cardiac diseases, however, among these the genes involved in the synthesis and metabolism of serum lipids have been subject of intense research due to their clinical significance and their ability to be quantified from the serum. We therefore selected variants in the genes that affect two key serum lipids namely triglycerides and high density lipoprotein cholesterol with the aim to check whether any change in the genes results in the change of the serum levels of these lipids.

The association of LPL polymorphisms with lipid levels has been controversial [6]. Most of the SNPs studied in this gene are rare and limited either to single families or are restricted to isolated geographic regions, whereas, common variants show differences in allele frequencies among different populations [26]. In our study, minor allele (G) of the SNP LPL rs328 was found to be protective as its presence lowers TG and raises HDL-C. The common allele (C) was the risk allele. The carriers of risk allele had higher plasma TG and lower HDL-C levels. However, a significant difference in allele frequencies was not observed in cases than controls. Since the LPL rs328 causes a protein change towards the C terminal, hence it affects the legend function of the protein leading to enhanced uptake of lipoproteins at cellular receptors [27]. Hence this is a gain in function change and such changes are rare in nature and prevent from cardiovascular events [28]. Such mutations are blessing in present eras where people are living with a large number of CAD risk factors. Our results were also inconsistent with many other studies [29–33]. The risk allele of LPL rs1801177 was completely absent in the subjects under study. In European people, the frequency of this allele has been reported to vary from 1.6 – 6.7% [34]. In another meta-analysis of 89 studies, the average frequency of risk allele was less than 3% ranging from <0.1 – 8% [10]. We previously have reported the RAF of 0.01% in another study group from Pakistan [35].

We have replicated the effect of ApoA5 rs662799 in Pakistani subjects and similar results were reported in a study conducted in India where the RAF was associated with 19% increase in serum TG levels [36]. Similar TG raising effect of this SNP has also been reported in Chinese people [15, 17]. Since the allele frequencies of this SNP show interethnic variations [37], its association with biochemical markers may also vary in different ethnicities. Due to the presence of protective variants, black population whether from Caribbean or South African ancestries have low TG, high HDL-C with less CAD mortality than the white populations. Whereas South Asian immigrants to Europe exhibit high mortality rate associated with CAD secondary to raised serum TG and HDL-C [38]. Similar effect of this variant on blood lipids have been documented in Caucasians, African American and Hispanics [16, 39–41].

The presence of C allele of CETP rs708272 decreased HDL-C and increased TG levels, both are CAD risk factors. The results were in agreement with another prospective study where each C allele was associated with 3.1 mg/dl decrease in HDL-C concentration and a 24% increase in CAD risk [42]. In a meta-analysis of 98 published studies, the protective allele T was associated with lower CETP mass and activity and higher mean HDL-C levels [43]. Similar results have also been reported in Chinese people [44]. The role of CETP protein in lipid metabolism, though largely studied, is still controversial. According to Thompson et al. (2008), the CETP polymorphisms were associated with moderate inhibition of CETP activity, increased HDL-C concentration and decreasing CAD risk. However, some other studies have reported greater CAD risk associated with decreased CETP concentration [45, 46]. Furthermore, the effect of this SNP is also modulated by environmental effects [21] and the LD pattern of the tag SNPs in CETP gene is also different in Asians compared to Europeans [20].

The study had some limitations, it included only four polymorphisms in the lipid metabolism genes, inclusion of other polymorphisms in a large number of genes affecting serum lipid traits may have improved the results of the study and should be considered in future. Furthermore, the study had relatively small sample size therefore future studies with even larger sample size and more variants included, should be done in order to confirm the findings of the current study. The implications of the study include providing the baseline information about the polymorphisms in the Pakistani population and the probable mechanism through which these variants may affect TG and HDL-metabolism.

Conclusion

In conclusion, the SNPs were associated with blood TG and HDL-C levels additively but we could not find a significant high risk allele frequency in the cases than the controls. However, because the SNPs had small effect size and association with CAD was also modest, we tried to examine their combined effect on CAD risk and TG and HDL-C levels. The association with CAD still remained robust; however, the combined effect of the SNPs was highly significant on TG and HDL-C levels. This information can be used to establish panels comprising of common variants with modest effect on the serum lipid traits to establish protocols for identifying the genetic component responsible for changes in serum lipid chemistry in a routine clinical setting.

Abbreviations

ANOVA:

Analysis of variance

CAD:

Coronary artery disease

CETP:

Cholesterol ester transfer protein

CM:

Chylomicrons

HDL-C:

High density lipoprotein cholesterol

IDL:

Intermediate density lipoprotein

LDL:

Low density lipoprotein

LPL:

Lipoprotein lipase

OR:

Odds ratio

SDS:

Sequence detection software

SNPs:

Single nucleotide polymorphisms

SPSS:

Statistical package for social sciences

TG:

Triglycerides

References

  1. Merkel M, Eckel RH, Goldberg IJ. Lipoprotein lipase genetics, lipid uptake, and regulation. J Lipid Res. 2002;43(12):1997–2006.

    Article  CAS  PubMed  Google Scholar 

  2. Martin-Rehrmann MD, Cho HS, Rebeck GW. Lack of association of two lipoprotein lipase polymorphisms with Alzheimer's disease. Neurosci Lett. 2002;328(2):109–12.

    Article  CAS  PubMed  Google Scholar 

  3. Eckel RH. Lipoprotein lipase. A multifunctional enzyme relevant to common metabolic diseases. New Engl J Med. 1989;320(16):1060–8.

    Article  CAS  PubMed  Google Scholar 

  4. Corella D, Guillén M, Sáiz C, Portolés O, Sabater A, Folch J, et al. Associations of LPL and APOC3 gene polymorphisms on plasma lipids in a Mediterranean population: interaction with tobacco smoking and the APOE locus. J Lipid Res. 2002;43(3):416–27.

    CAS  PubMed  Google Scholar 

  5. Borén J, Matikainen N, Adiels M, Taskinen M-R. Postprandial hypertriglyceridemia as a coronary risk factor. Clin Chim Acta. 2014;431:131–42.

    Article  PubMed  Google Scholar 

  6. Rebhi L, Kchok K, Omezzine A, Kacem S, Rejeb J, et al. Six lipoprotein lipase gene polymorphisms, lipid profile and coronary stenosis in a Tunisian population. Mol Biol Rep. 2012;39(11):9893–901.

    Article  CAS  PubMed  Google Scholar 

  7. Ariza M-J, Sánchez-Chaparro M-Á, Barón F-J, Hornos A-M, Calvo-Bonacho E, et al. Additive effects of LPL, APOA5 and APOE variant combinations on triglyceride levels and Hypertriglyceridemia: results of the ICARIA genetic sub-study. BMC Med Genet. 2010;11:1–10.

    Article  Google Scholar 

  8. Takagi A, Ikeda Y. Genetic diagnosis on hypertriglyceridemia-analysis for LPL gene mutations. Jpn J Clin Med. 2013;71(9):1569–76.

    Google Scholar 

  9. Tan A, Sun J, Xia N, Qin X, Hu Y, Zhang S, et al. A genome-wide association and gene–environment interaction study for serum triglycerides levels in a healthy Chinese male population. Hum Mol Genet. 2012;21(7):1658–64.

    Article  CAS  PubMed  Google Scholar 

  10. Sagoo GS, Tatt I, Salanti G, Butterworth AS, Sarwar N, van Maarle M, et al. Seven lipoprotein lipase gene polymorphisms, lipid fractions, and coronary disease: a HuGE association review and meta-analysis. Am J Epidemiol. 2008;168(11):1233–46.

    Article  PubMed  Google Scholar 

  11. Shimo-Nakanishi Y, Urabe T, Hattori N, Watanabe Y, Nagao T, Yokochi M, et al. Polymorphism of the lipoprotein lipase gene and risk of atherothrombotic cerebral infarction in the Japanese. Stroke. 2001;32(7):1481–6.

    Article  CAS  PubMed  Google Scholar 

  12. Li R-K, Guo J. Single nucleotide variances can account for loss of microRNA function: the emerging cross talk between genetics and epigenetics. J Am Coll Cardiol. 2014;64(3):278–80.

    Article  CAS  PubMed  Google Scholar 

  13. Garelnabi M, Lor K, Jin J, Chai F, Santanam N. The paradox of ApoA5 modulation of triglycerides: evidence from clinical and basic research. Clin Biochem. 2013;46(1):12–9.

    Article  CAS  PubMed  Google Scholar 

  14. van der Vliet HN, Schaap FG, Levels JH, Ottenhoff R, Looije N, Wesseling JG, et al. Adenoviral overexpression of apolipoprotein AV reduces serum levels of triglycerides and cholesterol in mice. Biochem Biophys Res Commun. 2002;295(5):1156–9.

    Article  CAS  PubMed  Google Scholar 

  15. Zhu W-f, Wang C-l, Liang L, Shen Z, Fu J-f, et al. Triglyceride-raising APOA5 genetic variants are associated with obesity and non-HDL-C in Chinese children and adolescents. Lipids Health Dis. 2014;13(1):1–7.

    Article  PubMed Central  Google Scholar 

  16. Aouizerat BE, Kulkarni M, Heilbron D, Drown D, Raskin S, Pullinger CR, et al. Genetic analysis of a polymorphism in the human apoA-V gene effect on plasma lipids. J Lipid Res. 2003;44(6):1167–73.

    Article  CAS  PubMed  Google Scholar 

  17. Jiang CQ, Liu B, Cheung BM, Lam TH, Lin JM, et al. A single nucleotide polymorphism in APOA5 determines triglyceride levels in Hong Kong and Guangzhou Chinese. Eur J Hum Genet. 2010;18(11):1255–60.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Barter JP, Brewer HB, Chapman MJ, Hennekens CH, Rader DJ, et al. Cholesteryl ester transfer protein a novel target for raising HDL and inhibiting atherosclerosis. Arterioscler Thromb Vasc Biol. 2003;23(2):7.

    Article  Google Scholar 

  19. Barter PJ, Hopkins G, Calvert G. Transfers and exchanges of esterified cholesterol between plasma lipoproteins. Biochem J. 1982;208:1–7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Schierer A, Been LF, Ralhan S, Wander GS, Aston CE, Sanghera DK. Genetic variation in cholesterol ester transfer protein (CETP), serum CETP activity, and coronary artery disease (CAD) risk in Asian Indian diabetic cohort. Pharmacogenet Genom. 2012;22(2):95–104.

    Article  CAS  Google Scholar 

  21. Yilmaz H, İsbir T, Agachan B, Ermis KZ. Effects of cholesterol ester transfer protein Taq1B gene polymorphism on serum lipoprotein levels in Turkish coronary artery disease patients. Cell Biochem Funct. 2005;23(1):23–8.

    Article  CAS  PubMed  Google Scholar 

  22. Kathiresan S, Willer CJ, Peloso GM, Demissie S, Musunuru K, Schadt EE, et al. Common variants at 30 loci contribute to polygenic dyslipidemia. Nat Genet. 2009;41(1):56–65.

    Article  CAS  PubMed  Google Scholar 

  23. Shahid SU, Rehman A, Hasnain S. Role of a common variant of Fat mass and obesity associated (FTO) gene in obesity and coronary artery disease in subjects from Punjab, Pakistan: a case control study. Lipids Health Dis. 2016;15(1):1.

    Article  Google Scholar 

  24. Shahid SU, Cooper JA, Beaney KE, Li K, Rehman A, Humphries SE. Effect of SORT1, APOB and APOE polymorphisms on LDL-C and coronary heart disease in Pakistani subjects and their comparison with Northwick park heart study II. Lipids Health Dis. 2016;15(1):1.

    Article  Google Scholar 

  25. Johnson AF. Statistics in medicine T. Colton. Little, Brown and Company, Boston, Mass. 02106, 1974, xii + 372 pp. Paperback. Clin Chem. 1975;21(6):789–90.

    Google Scholar 

  26. Mo X, Liu X, Wang L, Lu X, Chen S, Li H, et al. Association of lipoprotein lipase polymorphism rs2197089 with serum lipid concentrations and LPL gene expression. J Hum Genet. 2013;58(3):160–4. Epub 2013/01/25.

    Article  CAS  PubMed  Google Scholar 

  27. Wittrup HH, Tybjærg-Hansen A, Nordestgaard BG. Lipoprotein lipase mutations, plasma lipids and lipoproteins, and risk of ischemic heart disease a meta-analysis. Circulation. 1999;99(22):2901–7.

    Article  CAS  PubMed  Google Scholar 

  28. Rip J, Nierman MC, Ross CJ, Jukema JW, Hayden MR, Kastelein JJ, et al. Lipoprotein lipase S447X a naturally occurring gain-of-function mutation. Arterioscler Thromb Vasc Biol. 2006;26(6):1236–45.

    Article  CAS  PubMed  Google Scholar 

  29. Ahn Y, Kamboh M, Hamman R, Cole S, Ferrell R. Two DNA polymorphisms in the lipoprotein lipase gene and their associations with factors related to cardiovascular disease. J Lipid Res. 1993;34(3):421–8.

    CAS  PubMed  Google Scholar 

  30. Hokanson JE. Lipoprotein lipase gene variants and risk of coronary disease: a quantitative analysis of population-based studies. Int J Clin Lab Res. 1997;27(1):24–34.

    Article  CAS  PubMed  Google Scholar 

  31. Humphries SE, Nicaud V, Margalef J, Tiret L, Talmud PJ. Lipoprotein lipase gene variation is associated with a paternal history of premature coronary artery disease and fasting and postprandial plasma triglycerides :The European Atherosclerosis Research Study (EARS). Arterioscler Thromb Vasc Biol. 1998;18(4):526–34.

    Article  CAS  PubMed  Google Scholar 

  32. Garenc C, Pérusse L, Gagnon J, Chagnon YC, Bergeron J, Després J-P, et al. Linkage and association studies of the lipoprotein lipase gene with postheparin plasma lipase activities, body fat, and plasma lipid and lipoprotein concentrations: the HERITAGE Family Study. Metabolism. 2000;49(4):432–9.

    Article  CAS  PubMed  Google Scholar 

  33. Mitchell RJ, Earl L, Bray P, Fripp YJ, Williams J. DNA polymorphisms at the lipoprotein lipase gene and their association with quantitative variation in plasma high-density lipoproteins and triacylglycerides. Hum Biol. 1994;66(3):383–97.

    CAS  PubMed  Google Scholar 

  34. Fisher MR, Humphries SE, Talmud PJ. Common variation in the lipoprotein lipase gene: effects on plasma lipids and risk of atherosclerosis. Atherosclerosis. 1997;135(2):145–59.

    Article  CAS  PubMed  Google Scholar 

  35. Beaney KE, Cooper JA, Shahid SU, Ahmed W, Qamar R, Drenos F, et al. Clinical utility of a coronary heart disease risk prediction gene score in UK healthy middle aged men and in the Pakistani population. PLoS One. 2015;10(7):e0130754.

    Article  PubMed  PubMed Central  Google Scholar 

  36. Chandak RG, Ward KJ, Yajnik CS, Pandit AN, Bavdekar A, et al. Triglyceride associated polymorphisms of the APOA5 gene have very different allele frequencies in Pune, India compared to Europeans. BMC Med Genet. 2006;7(1):1–6.

    Article  Google Scholar 

  37. Lai C-Q, Tai E-S, Tan CE, Cutter J, Chew SK, Zhu Y-P, et al. The APOA5 locus is a strong determinant of plasma triglyceride concentrations across ethnic groups in Singapore. J Lipid Res. 2003;44(12):2365–73.

    Article  CAS  PubMed  Google Scholar 

  38. Hall S, Talmud PJ, Cook DG, Wicks PD, Rothwell MJ, Strazzullo P, et al. Frequency and allelic association of common variants in the lipoprotein lipase gene in different ethnic groups: the Wandsworth Heart and Stroke Study. Genet Epidemiol. 2000;18(3):203–16.

    Article  CAS  PubMed  Google Scholar 

  39. Lee WJK, Ayyobi AF, Frohlich JJ, Hill JS. APOA5 gene polymorphism modulates levels of triglyceride, HDL cholesterol and FER HDL but is not a risk factor for coronary artery disease. Atherosclerosis. 2004;176(1):165–72.

    Article  CAS  PubMed  Google Scholar 

  40. Talmud PJ, Hawe E, Martin S, Olivier M, Miller GJ, Rubin EM, et al. Relative contribution of variation within the APOC3/A4/A5 gene cluster in determining plasma triglycerides. Hum Mol Genet. 2002;11(24):3039–46.

    Article  CAS  PubMed  Google Scholar 

  41. Martin S, Nicaud V, Humphries SE, Talmud PJ. Contribution of APOA5 gene variants to plasma triglyceride determination and to the response to both fat and glucose tolerance challenges. Biochim Biophys Acta. 2003;1637(3):217–25.

    Article  CAS  PubMed  Google Scholar 

  42. Ridker PM, Paré G, Parker AN, Zee RY, Miletich JP, Chasman DI. Polymorphism in the CETP gene region, HDL cholesterol, and risk of future myocardial infarction genomewide analysis among 18245 initially healthy women from the women’s genome health study. Circ Cardiovasc Genet. 2009;2(1):26–33.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  43. Thompson A, Di Angelantonio E, Sarwar N, Erqou S, Saleheen D, Dullaart RP, et al. Association of cholesteryl ester transfer protein genotypes with CETP mass and activity, lipid levels, and coronary risk. JAMA. 2008;299(23):2777–88.

    Article  CAS  PubMed  Google Scholar 

  44. Wang J, Wang LJ, Zhong Y, Gu P, Shao JQ, et al. CETP gene polymorphisms and risk of coronary atherosclerosis in a Chinese population. Lipids Health Dis. 2013;12(1):1–5.

    Article  Google Scholar 

  45. Bruce C, Sharp DS, Tall AR. Relationship of HDL and coronary heart disease to a common amino acid polymorphism in the cholesteryl ester transfer protein in men with and without hypertriglyceridemia. J Lipid Res. 1998;39(5):1071–8.

    CAS  PubMed  Google Scholar 

  46. Agerholm-Larsen B, Nordestgaard BG, Steffensen R, Jensen G, Tybjærg-Hansen A. Elevated HDL cholesterol is a risk factor for ischemic heart disease in white women when caused by a common mutation in the cholesteryl ester transfer protein gene. Circulation. 2000;101(16):1907–12.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgement

Higher education commission, Pakistan and British heart foundation laboratories, UK are acknowledged for research support.

Funding

SUS is funded by Higher Education Commission (HEC) of Pakistan under indigenous 5000 PhD scholarship programme (Bm6-131) and international research support initiative programme scheme (IRSIP 24 BMS 41). SEH is British heart foundation professor and he and JC are supported by the British Heart Foundation (RG 2008/008) and also funding from the Department of National Institute for Health Research (NIHR) Biomedical Research Centres funding scheme.

Availability of data and materials

Corresponding author can be contacted for that.

Authors’ contribution

SUS, Designed the study, performed the experiments, analysed the results outlined and wrote the manuscript. NAS, helped in experimental work. JAC helped in statistical analysis. AR helped in manuscript writing and supervised the study. SEH, Provided logistic support, designed and supervised the project. All authors read and approved the final manuscript.

Competing interests

Authors declare that they do not have any competing interests.

Consent for publication

No such data included.

Ethics approval and consent to participate

Not applicable.

Statement of ethical approval

The participants gave a prior written informed consent. The study was approved by the institutional ethical committee, university of the Punjab, Lahore.

Author information

Authors and Affiliations

  1. Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore, Pakistan, 54590

    Saleem Ullah Shahid, N.A. Shabana & Abdul Rehman

  2. Centre of Cardiovascular Genetics, British Heart Foundation Laboratories, University College London, London, WC1E6JF, UK, England

    Jackie A. Cooper & Steve E. Humphries

Authors
  1. Saleem Ullah Shahid
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  2. N.A. Shabana
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  3. Jackie A. Cooper
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  4. Abdul Rehman
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  5. Steve E. Humphries
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Corresponding author

Correspondence to Saleem Ullah Shahid.

Additional files

Additional file 1: Table S1.

List of primers and probes used in KASPar assay. (DOCX 11 kb)

Additional file 2: Table S2.

Sequence of primers used in PCR. (DOCX 11 kb)

Additional file 3: Table S3.

Basic features of SNPs under study. (DOCX 13 kb)

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Shahid, S.U., Shabana, N., Cooper, J.A. et al. Common variants in the genes of triglyceride and HDL-C metabolism lack association with coronary artery disease in the Pakistani subjects. Lipids Health Dis 16, 24 (2017). https://doi.org/10.1186/s12944-017-0419-4

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  • DOI: https://doi.org/10.1186/s12944-017-0419-4

Keywords

Lipids in Health and Disease

ISSN: 1476-511X