Graphical representation of the trend and spread of the hepatic microarray data. Blue dots indicate the highest fold change for each probe set on the Affymetrix Mu11K chip, where the highest fold change was identified by examining all fold changes in every possible pairwise comparison between diets. Those probe sets that were identified as absent (by Affymetrix software) across all dietary treatments were treated as unchanged and therefore not considered in the limit fold change (LFC) selection model. The trend within this dataset indicates that as absolute expression increases (along the x-axis), fold changes decrease, i.e. the chance of seeing large fold changes with highly expressed genes is minimal. The 5% LFC line of best fit (red line) takes this observed trend into account and identifies the upper 5% of those probe sets with the greatest fold changes within predefined bins (model parameters fully explained in ). The 329 probe sets that lie above the 5% LFC line are those genes defined as differentially regulated. Green circles represent the hepatic genes discussed in the current manuscript and black squares represent those genes validated by real time PCR. When examining the spread of the genes discussed and validated, it is apparent that genes were selected across the entire range of absolute values and not concentrated in one area. Furthermore, as the validation data (which had 86% concordance with microarray data; see ) lies across the entire range of absolute expression levels, a high degree of confidence is established for the genes discussed within the present manuscript.