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Table 1 Impact of Different Normalizations on the Quantification of Anandamide in Brain, and in Human Cells

From: Quantification of anandamide content in animal cells and tissues: the normalization makes the difference

Anandamide content

Rat brain

Mouse brain

Neuroblastoma CHP100 cells

Lymphoma U937 cells

pmol/mg protein

350 ± 70

400 ± 75

340 ± 65

400 ± 70

pmol/mg DNA

700 ± 98

800 ± 160

380 ± 70

440 ± 80

pmol/mg Pia

1.4 ± 0.3

1.6 ± 0.3

2.1 ± 0.4

3.3 ± 0.7

pmol/mg FWb

14 ± 5.6

16 ± 6.4

27 ± 9.7

28 ± 10.5

pmol/106 cells

N.D.c

N.D.c

19 ± 4

18 ± 4

  1. Values are reported as the mean ± S.D. of 6 to 8 independent determinations, each performed in duplicate. aPi, lipid phosphorus; bFW, fresh weight; cN.D., not determined.
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Lipids in Health and Disease

ISSN: 1476-511X