| PPARα | PPARγ | PPARδ |
---|
FA | 100 μM | 200 μM | 100 μM | 200 μM | 100 μM | 200 μM |
18:2n-6 | 23 | 43 | 8 | 12 | 11 | 13 |
20:3n-6 | 17 | 19 | 3 | 3 | 20 | 4 |
20:3n-3 | 19 | 49 | 18 | 30 | 53 | 67 |
5,11,14 20:3 | 16 | 35 | 22 | 27 | 28 | 56 |
9,11/10,12 18:2 | 76 | 98 | 11 | 14 | 15 | 32 |
22:6n-3 | 65 | 574 | 50 | 298 | 140 | 428 |
- Luciferase activity in HeLa cells transfected with human PPARα, δ and γ cDNAs measured 24 h after addition of unesterified FA at indicated concentration. Values represent % of transactivation values obtained in presence of the positive control Wy14,643 at 10 μM for PPARα, and of BRL49653 at 1 μM for PPARγ and PPARδ, taken as 100%. Significant activation is considered >50% of Wy14,643 for PPARα; >75% of BRL49653 for PPARγ and >100% of BRL49653 for PPARδ. Under the assay conditions employed, inhibition values could not be determined. Values represent mean of 3 replicates in independent experiments, with 4 replicates in each experiment.