| |
PPARα
|
PPARγ
|
PPARδ
|
|---|
|
FA
|
100 μM
|
200 μM
|
100 μM
|
200 μM
|
100 μM
|
200 μM
|
|
18:2n-6
|
23
|
43
|
8
|
12
|
11
|
13
|
|
20:3n-6
|
17
|
19
|
3
|
3
|
20
|
4
|
|
20:3n-3
|
19
|
49
|
18
|
30
|
53
|
67
|
|
5,11,14 20:3
|
16
|
35
|
22
|
27
|
28
|
56
|
|
9,11/10,12 18:2
|
76
|
98
|
11
|
14
|
15
|
32
|
|
22:6n-3
|
65
|
574
|
50
|
298
|
140
|
428
|
- Luciferase activity in HeLa cells transfected with human PPARα, δ and γ cDNAs measured 24 h after addition of unesterified FA at indicated concentration. Values represent % of transactivation values obtained in presence of the positive control Wy14,643 at 10 μM for PPARα, and of BRL49653 at 1 μM for PPARγ and PPARδ, taken as 100%. Significant activation is considered >50% of Wy14,643 for PPARα; >75% of BRL49653 for PPARγ and >100% of BRL49653 for PPARδ. Under the assay conditions employed, inhibition values could not be determined. Values represent mean of 3 replicates in independent experiments, with 4 replicates in each experiment.
