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Table 3 Induction of transactivation of PPARs

From: Epidermal anti-Inflammatory properties of 5,11,14 20:3: Effects on mouse ear edema, PGE2 levels in cultured keratinocytes, and PPAR activation

  PPARα PPARγ PPARδ
FA 100 μM 200 μM 100 μM 200 μM 100 μM 200 μM
18:2n-6 23 43 8 12 11 13
20:3n-6 17 19 3 3 20 4
20:3n-3 19 49 18 30 53 67
5,11,14 20:3 16 35 22 27 28 56
9,11/10,12 18:2 76 98 11 14 15 32
22:6n-3 65 574 50 298 140 428
  1. Luciferase activity in HeLa cells transfected with human PPARα, δ and γ cDNAs measured 24 h after addition of unesterified FA at indicated concentration. Values represent % of transactivation values obtained in presence of the positive control Wy14,643 at 10 μM for PPARα, and of BRL49653 at 1 μM for PPARγ and PPARδ, taken as 100%. Significant activation is considered >50% of Wy14,643 for PPARα; >75% of BRL49653 for PPARγ and >100% of BRL49653 for PPARδ. Under the assay conditions employed, inhibition values could not be determined. Values represent mean of 3 replicates in independent experiments, with 4 replicates in each experiment.