Expression of M1 and M2 macrophage polarized markers in human monocyte-derived macrophages. A. TNFα, IL1β, and IL6 gene expression as markers of M1, and MRC1 gene expression as a marker of M2 were analyzed by RT-PCR. Each panel shows data from one of 3 representative experiments. B. Surface expression of CD86 (M1 marker) and mannose receptor (MR) (M2 marker) were analyzed by flow cytometry. The level of CD86 expression was higher in M1 than in M2 macrophages, while the expression level of MR was higher in M2 than in M1 macrophages.