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Table 1 Genes upregulated by oxLDL in polarized macrophages

From: Different responses to oxidized low-density lipoproteins in human polarized macrophages

The top 30 genes upregulated by oxLDL in M0
Gene Symbol Ref Seq ID M0 M1 M2 NCBI official full name
IL8 NM_000584 6.200 3.200 5.177 interleukin 8
CCL1 NM_002981 6.028 0.937 - chemokine (C-C motif) ligand 1
SERPINB2 NM_002575 5.485 - - serpin peptidase inhibitor, clade B (ovalbumin), member 2
IL1B NM_000576 5.162 2.700 2.684 interleukin 1, beta
PTGS2 NM_000963 4.985 - - prostaglandin-endoperoxide synthase 2
TM4SF1 NM_014220 4.836 - 1.238 transmembrane 4 L six family member 1
CCL19 NM_006274 4.834 0.368 - chemokine (C-C motif) ligand 19
POPDC3 NM_022361 4.557 1.491 - popeye domain containing 3
INHBA NM_002192 4.476 2.335 - inhibin, beta A
CKB NM_001823 4.378 0.920 2.107 creatine kinase, brain
IFIT1 NM_001001887 4.152 0.717 5.734 interferon-induced protein with tetratricopeptide repeats 1
CCL5 NM_002985 4.075 -0.218 1.616 chemokine (C-C motif) ligand 5
IFIT5 NM_012420 4.039 - - interferon-induced protein with tetratricopeptide repeats 5
TNFAIP6 NM_007115 4.033 0.019 - tumor necrosis factor, alpha-induced protein 6
TRIM16 NM_006470 3.990 2.992 3.453 tripartite motif-containing 16
ADM NM_001124 3.973 4.807 3.202 adrenomedullin
CCL4L2 NM_207007 3.905 1.321 0.448 chemokine (C-C motif) ligand 4-like 2
ISG20 NM_002201 3.839 0.952 4.442 interferon stimulated exonuclease gene 20kDa
EREG NM_001432 3.726 2.793 - epiregulin
CSF2 NM_000758 3.644 1.605 0.724 colony stimulating factor 2 (granulocyte-macrophage)
RHOF NM_019034 3.638 - 3.020 ras homolog gene family, member F (in filopodia)
GSTM3 NM_000849 3.632 2.635 2.543 glutathione S-transferase mu 3 (brain)
AKR1C3 NM_003739 3.497 2.490 3.432 aldo-keto reductase family 1, member C3
CYP7B1 NM_004820 3.482 - - cytochrome P450, family 7, subfamily B, polypeptide 1
CCL4 NM_002984 3.466 0.742 -0.123 chemokine (C-C motif) ligand 4
CCL24 NM_002991 3.433 0.413 - chemokine (C-C motif) ligand 24
NT5E NM_002526 3.406 - - 5'-nucleotidase, ecto (CD73)
IFI44L NM_006820 3.325 -0.074 4.474 interferon-induced protein 44-like
COX11 NM_004375 3.306 1.946 1.111 COX11 cytochrome c oxidase assembly homolog (yeast)
NOL12 NM_024313 3.281 0.937 1.313 nucleolar protein 12
The top 30 genes upregulated by oxLDL in M1
Gene Symbol Ref Seq ID M0 M1 M2 NCBI official full name
ADM NM_001124 3.973 4.807 3.202 adrenomedullin
S1RT5 NM_012241 3.039 3.486 2.137 sirtuin 5
CXCR5 NM_032966 1.986 3.437 1.815 chemokine (C-X-C motif) receptor 5
KLHL21 NM_014851 2.380 3.324 - kelch-like 21 (Drosophila)
IL8 NM_000584 6.200 3.200 5.177 interleukin 8
DPYSL3 NM_001387 3.064 3.192 3.497 dihydropyrimidinase-like 3
RAB43 NM_198490 1.264 3.003 3.324 RAB43, member RAS oncogene familyprovided
TRIM16 NM_006470 3.990 2.992 3.453 tripartite motif-containing 16
C1orf66 NM_015997 0.382 2.970 1.953 chromosome 1 open reading frame 66
PIP4K2A NM_005028 2.325 2.967 3.127 phosphatidylinositol-5-phosphate 4-kinase, type II, alpha
AGAP3 NM_031946 1.289 2.945 1.091 ArfGAP with GTPase domain, ankyrin repeat and PH domain 3
INPP4A NM_001566 0.129 2.872 1.872 inositol polyphosphate-4-phosphatase, type I, 107kDa
DAGLA NM_006133 1.545 2.834 2.807 diacylglycerol lipase, alpha
EREG NM_001432 3.726 2.793 - epiregulin
AGAP11 NM_133447 0.971 2.791 2.318 ankyrin repeat and GTPase domain Arf GTPase activating protein 11
ADAMTS10 NM_030957 0.683 2.773 0.952 ADAM metallopeptidase with thrombospondin type 1 motif, 10
KCP NM_199349 1.269 2.746 1.861 kielin/chordin-like protein
ZFAT NM_020863 1.758 2.743 2.002 zinc finger and AT hook domain containing
LZTR1 NM_006767 1.092 2.714 3.134 leucine-zipper-like transcription regulator 1
MON1B NM_014940 0.234 2.712 -0.754 MON1 homolog B (yeast)
IL1B NM_000576 5.162 2.700 2.684 interleukin 1, beta
STAM NM_003473 -0.222 2.697 -0.150 signal transducing adaptor molecule (SH3 domain and ITAM motif) 1
MAFG NM_032711 2.775 2.684 2.997 v-maf musculoaponeurotic fibrosarcoma oncogene homologG (avian)
OSGIN1 NM_182981 2.427 2.672 2.002 oxidative stress induced growth inhibitor 1
ZNF673 NM_017776 0.606 2.669 - zinc finger family member 673
IREB2 NM_004136 - 2.648 - iron-responsive element binding protein 2
GSTM3 NM_000849 3.632 2.635 2.543 glutathione S-transferase mu 3 (brain)
UBR2 NM_015255 2.160 2.619 2.371 ubiquitin protein ligase E3 component n-recognin 2
SFT2D3 NM_032740 1.146 2.577 1.609 SFT2 domain containing 3
FAM70A NM_017938 - 2.577 2.614 family with sequence similarity 70, member A
The top 30 genes upregulated by oxLDL in M2
Gene Symbol Ref Seq ID M0 M1 M2 NCBI official full name
IFIT2 NM_001547 2.559 - 5.893 interferon-induced protein with tetratricopeptide repeats 2
IFIT1 NM_001001887 4.152 0.717 5.734 interferon-induced protein with tetratricopeptide repeats 1
IL8 NM_000584 6.200 3.200 5.177 interleukin 8
MX2 NM_002463 2.482 0.562 4.552 myxovirus (influenza virus) resistance 2 (mouse)
IFI44L NM_006820 3.325 -0.074 4.474 interferon-induced protein 44-like
ISG20 NM_002201 3.839 0.952 4.442 interferon stimulated exonuclease gene 20kDa
IDO1 NM_002164 - 0.623 4.335 indoleamine 2,3-dioxygenase 1
PLSCR1 NM_021105 2.002 -0.179 4.119 phospholipid scramblase 1
IFIT3 NM_001549 2.306 -0.483 3.943 interferon-induced protein with tetratricopeptide repeats 3
SGPP2 NM_152386 3.229 - 3.626 sphingosine-1-phosphate phosphatase 2
OR9I1 NM_001005211 2.923 1.683 3.574 olfactory receptor, family 9, subfamily I, member 1
VNN1 NM_004666 0.568 0.896 3.569 vanin 1
DPYSL3 NM_001387 3.064 3.192 3.497 dihydropyrimidinase-like 3
TRIM16 NM_006470 3.990 2.992 3.453 tripartite motif-containing 16
EPSTI1 NM_033255 2.678 -0.642 3.439 epithelial stromal interaction 1 (breast)
AKR1C3 NM_003739 3.497 2.490 3.432 aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II)
RAB43 NM_198490 1.264 3.003 3.324 RAB43, member RAS oncogene family
APOL6 NM_030641 1.925 0.723 3.291 apolipoprotein L, 6
USP18 NM_017414 1.686 0.052 3.288 ubiquitin specific peptidase 18
ADM NM_001124 3.973 4.807 3.202 adrenomedullin
RSAD2 NM_080657 1.858 0.083 3.194 radical S-adenosyl methionine domain containing 2
LZTR1 NM_006767 1.092 2.714 3.134 leucine-zipper-like transcription regulator 1
PIP4K2A NM_005028 2.325 2.967 3.127 phosphatidylinositol-5-phosphate 4-kinase, type II, alpha
CSF2RA NM_172247 1.683 2.109 3.115 colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)
TNFRSF9 NM_001561 2.899 1.702 3.100 tumor necrosis factor receptor superfamily, member 9
ARHGAP44 NM_014859 1.136 1.919 3.053 Rho GTPase activating protein 44
RHOF NM_019034 3.638 - 3.020 ras homolog gene family, member F (in filopodia)
CWF19L1 NM_018294 1.271 2.098 3.005 CWF19-like 1, cell cycle control (S. pombe)
MAFG NM_032711 2.775 2.684 2.997 v-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian)
TNFRSF4 NM_003327 1.815 1.376 2.974 tumor necrosis factor receptor superfamily, member 4
  1. Non-polarized (M0) and polarized M1 and M2 macrophages were treated with or without oxLDL for 6 h. The changes in gene expression were analyzed by cDNA microarray analysis as described in the Materials and methods section. The top 30 upregulated genes in each subset of macrophages are listed. The values denote fold changes (log2 ratio) of normalized intensities during oxLDL treatment. Letters in boldface indicate genes that were also upregulated by more than 2-fold in the two other subsets. "-" indicates genes that did not show significantly altered expression. Twenty-eight of the top 30 upregulated genes in M2 macrophages (93% of the top 30 genes) were also upregulated in M0, while 15 genes in the top 30 upregulated genes in M2 macrophages were upregulated in M1 cells.