Mass accumulation of male C57bBl/6 mice exposed to a high fat diet with or without supplements. 1a) Animals in the phytosterol+ascorbic acid (PSAA) group exhibited a reduction in mass accumulation by week 2 and the difference became statistically significant by week 7 of the study (n = 7; ANOVA p < 0.05). 1b) Mass accumulation over time for C57Bl/6 animals consuming a high fat diet with ascorbic acid and phytosterol supplementation (this study) in comparison to animals consuming a high fat control diet with or without disodium ascorbyl phytostanyl phosphate(17). Mice chronically exposed to a high fat diet supplemented with 2% w/w phytostanol esterified with ascorbic acid show a greater decrease in mass accumulation than those consuming a high fat diet supplemented with unesterified phytosterols and ascorbic acid (1% w/w of each component). * indicates data obtained from this study.