Figure 2
From: Inflammatory stress exacerbates ectopic lipid deposition in C57BL/6J mice
Effects of chronic systemic inflammation on lipid accumulation in liver, muscle and white adipose tissue from C57BL/6J mice . Mice fed with normal chow diet (NCD), NCD plus casein injection (NCD+Casein), high fat diet (HFD) and HFD plus casein injection (HFD+Casein) were culled 18 weeks after first injection. The lipid accumulation in tissues was checked by Oil Red O staining (A, C, E, original magnification × 400) using 5-μm-thick sections and quantitative lipid measurements of tissues (B, D, F). The concentrations of TG and FFA in tissues of C57BL/6J mice were measured as described in Materials and Methods and results represent the mean ± SD (n = 6), * P < 0.05 versus NCD, †P < 0.05 versus HFD. (A (I-IV)) Oil Red O staining of liver. (B) The concentrations of TG (left) and FFA (right) in liver. (C (I-IV)) Oil Red O staining of muscle. (D) The concentrations of TG (left) and FFA (right) in muscle. (E (I-IV)) Oil Red O staining of white adipose tissue. (F) The concentrations of TG (left) and FFA (right) in white adipose tissue.