Metabolic activity in palmitate-exposed INS-1E cells. Cells were cultured for 48 hours in the presence of 0.5 mM palmitate at indicated glucose (Glu) concentrations. AICAR (1 mM; Aic) and etomoxir (0.2 mM; Et) were added as indicated. After culture, metabolic activity was measured using the MTT assay. INS-1E cells cultured at 11 mM glucose alone were considered as control. Results are means ± SEM of 3 independent experiments. *P < 0.05 compared to control.