p-ACC/ACC ratio (A) and FAS expression level (B) in palmitate-exposed INS-1E cells (white bars), MIN6 cells (grey bars) and human islets (black bars). The islets and cells were cultured for 48 hours in the presence of 0.5 mM palmitate at indicated glucose (Glu) concentrations. INS-1E cells cultured at 11 mM glucose, MIN6 cells at 25 mM glucose and human islets at 5.5 mM glucose were considered as controls. After culture, protein levels of p-ACC, ACC and FAS were measured. Results are means ± SEM of 3-4 independent experiments. *P < 0.05 compared to control.