Effect of palmitoleic acid on hepatic steatosis in KK-Ay mice. Animals were orally administered vehicle (control), 300 mg/kg of palmitic acid, or 300 mg/kg palmitoleic acid on a daily basis for 4 weeks. Graphs depicting neutral lipid accumulation in liver tissue are shown. (A) Hepatic triglyceride concentration. Values are means ± SE, n = 10. Means in a row with superscripts without common letter are different, p < 0.05. C16:0, palmitic acid; C16:1, palmitoleic acid. (B) Oil red O staining of liver sections from control (I), palmitic acid (II), and palmitoleic acid (III) groups of KK-Aymice. Images are shown at 20× magnification. Scale bar = 100 μm.