SDS-PAGE and Native-PAGE of the SCP. (A) Analysis of purified SCP by SDS-PAGE (13%). Lane 1, molecular mass markers (Pharmacia); lane 2, SCP solution (300 μg) obtained after DEAE-Sephadex chromatography; lane 3, SCP solution (60 μg) obtained after Sephadex G-100 chromatography; lane 4, SCP solution (40 μg) obtained after SP-Sepharose chromatography; lane 5, purified SCP obtained after Sephadex G-50 chromatography (20 μg). The gel was stained with Coomassie blue. (B) Native-PAGE of the purified enzyme, protein stained by Coomassie blue (lane 1); zymogram of the purified chymotrypsin SCP (lane 2).