Effects of temperature. (A) Effects of temperature on SCP and BCP activities. (B) Effects of temperature on SCP and BCP stabilities. Enzyme activity was tested at various temperatures using casein as substrate at pH 9 for the SCP and at pH 7.5 for the BCP in the presence of 4 mM CaCl2. Residual enzyme activity was determined after 15 min in incubation and the initial activity before incubation was taken as 100%.