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Table 1 Flow sheet of scorpion chymotrypsin-like protease purification.

From: A new chymotrypsin-like serine protease involved in dietary protein digestion in a primitive animal, Scorpio maurus: purification and biochemical characterization

Purification step Total activity* (U) × 103 Protein (mg) Specific activity (U/mg) Activity recovery (%) Purification factor
Extract of SCP (pH 8) 220 ± 47 925.80 ± 4.68 237.60 ± 8.90 100 1
DEAE-Sephadex 179.25 ± 12 203.95 ± 5.90 878.90 ± 25.60 81 ± 5.70 3.70 ± 0.12
(NH 4 ) 2 SO 4 Precipitation (75%) 164.80 ± 8.90 87 ± 3.78 1894.20 ± 98.70 91.90 ± 3.70 7.97 ± 0.43
Sephadex G-100 chromatography 103.90 ± 5.40 15.80 ± 0.87 6575.90 ± 578 63 ± 4.06 27.67 ± 0.28
SP-Sepharose chromatography 58.23 ± 1.09 0.98 ± 0.23 59418 ± 320 56 ± 0.95 250 ± 2.70
Sephadex G-50 chromatography 26.25 ± 1.25 0.35 ± 0.12 75000 ± 540 45 ± 1.05 315.60 ± 6.90
  1. * One unit of protease activity was defined as the amount of enzyme, which liberated 1 μg tyrosine in 1 min at 50°C and at pH 9.