Inhibitors | Concentration (mM) | Residual activity (%) |
---|
None
| | 100 |
PMSF
| 1 | 18 |
| 5 | 7 |
DTNB
| 1 | 100 |
| 5 | 100 |
EDTA
| 1 | 98 |
| 5 | 60 |
| 10 | 40 |
- Enzyme activity measured in the absence of any inhibitor was taken as 100%. The remaining protease activity was measured after incubation of enzyme with each inhibitor at 4°C for 15 min.