LDLs loading induces the excess generation of ROS. The MIN6 cells were exposed to the LDLs with indicated concentrations for 48 hours. Then the cells were subjected to the incubation with 20 μM DCFHDA, for 30 minutes at 37°C, followed by fixation and mounting. The cell images were obtained with confocal laser microscope. Bar, 25 μm (a). Fluorescent intensities of DCF (2',7 ' -dichlorofluorescein) are expressed as fold increases of the of control level (b). Mean ± SD (n = 20). *: p < 0.05 vs. the levels of control. #: p < 0.05 vs. the levels of LDLs-loaded cells.