The LDLs loading induces accumulation of intracellular cholesterol. MIN6 cells were exposed to CLC with 250 times dilution or 80 μM of LDLs in the growth medium. The medium was subjected directly to the extracellular cholesterol measure (a). The cells then were harvested and counted. The total lipids were extracted from cells and subjected to the intracellular cholesterol measurement (b). Mean ± SD (n = 3). *: p < 0.05 vs. the levels of control.