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Figure 3 | Lipids in Health and Disease

Figure 3

From: Proteolytic cleavage of stingray phospholipase A2: Isolation and biochemical characterization of an active N-terminal form

Figure 3

(A): Remaining activity of SPLA2 incubated with trypsin and chymotrypsin. SPLA2 was incubated with chymotrypsin or trypsin at 30°C (20:1 w/w) as described in Material and methods section. The remaining activity of SPLA2 cleaved by chymotrypsin and by trypsin towards PC emulsion in the presence of 4 mM NaTDC and 8 mM CaCl2 was measured at various incubation times. Samples were withdrawn from the incubation mixtures at various times and analysed. (B): SDS-gel electrophoresis analysis of the tryptic cleavage of SPLA2 as a function of time. The gel was stained with Coomassie blue to reveal proteins. Lane 1, molecular mass markers; lane 2, SPLA2; lanes 3, after incubation of SPLA2 with trypsin for 30 h. (C): SDS-gel electrophoresis analysis of the chymotryptic cleavage of SPLA2 as a function of time. The gel was stained with Coomassie blue to reveal proteins. Lane 1, molecular mass markers; lane 2, SPLA2; lane 3, after incubation of SPLA2 with chymotrypsin for 24 h.

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