Filtration on HPLC column and SDS-PAGE (15%) of fragments resulting from chymotryptic cleavage of SPLA2. (A) An aliquot of the incubation mixture (0.1 mg in 0.1 ml) was applied to a HPLC column Bio-sil SEC-125 (300 mm × 7.8 mm) equilibrated in 0.1 M phosphate buffer pH 6.8 containing 0.15 M NaCl, elution was performed at room temperature within 20 min with the same buffer at a flow rate of 1 ml/min. Molecular mass markers were used to estimate the molecular masses of eluted proteins. The effluent was monitored at 280 nm. AU: arbitrary units. (B) SDS-PAGE (15%) of the active fraction eluted from HPLC filtration. The gel was stained with Comassie blue to reveal proteins.