Table 4 Apparent kinetic parameters of SPLA2, 12 kDa SPLA2 and DrPLA2
Vmax (U/mg) | Km (mM) | Kcat (s-1) | Kcat/Km (mM-1 s-1) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
PE | PC | PS | PE | PC | PS | PE | PC | PS | PE | PC | PS | |
SPLA2 | 2850 ± 200 | 750 ± 50 | 290 ± 15 | 13 ± 0.7 | 17.33 ± 0.5 | 20 ± 1.3 | 669 ± 5.2 | 187 ± 2.65 | 68 ± 2.7 | 51.5 ± 3.7 | 11.5 ± 0.5 | 3.4 ± 0.8 |
12 kDa SPLA2 | 2730 ± 150 | 700 ± 80 | 300 ± 17 | 13.8 ± 1 | 16.5 ± 0.9 | 22 ± 0.7 | 640.8 ± 7 | 146.3 ± 3.8 | 70.42 ± 3.3 | 47.4 ± 2.2 | 9.96 ± 0.3 | 3.2 ± 0.3 |
DrPLA2 | 2300 ± 120 | 600 ± 45 | 190 ± 12 | 16 ± 1.2 | 22 ± 0.9 | 28 ± 1.5 | 539.9 ± 10 | 140 ± 2.7 | 44.6 ± 1.9 | 33.74 ± 2.8 | 6.36 ± 0.7 | 2.03 ± 0.1 |