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Table 4 Apparent kinetic parameters of SPLA2, 12 kDa SPLA2 and DrPLA2

From: Proteolytic cleavage of stingray phospholipase A2: Isolation and biochemical characterization of an active N-terminal form

  Vmax (U/mg) Km (mM) Kcat (s-1) Kcat/Km (mM-1 s-1)
  PE PC PS PE PC PS PE PC PS PE PC PS
SPLA2 2850 ± 200 750 ± 50 290 ± 15 13 ± 0.7 17.33 ± 0.5 20 ± 1.3 669 ± 5.2 187 ± 2.65 68 ± 2.7 51.5 ± 3.7 11.5 ± 0.5 3.4 ± 0.8
12 kDa SPLA2 2730 ± 150 700 ± 80 300 ± 17 13.8 ± 1 16.5 ± 0.9 22 ± 0.7 640.8 ± 7 146.3 ± 3.8 70.42 ± 3.3 47.4 ± 2.2 9.96 ± 0.3 3.2 ± 0.3
DrPLA2 2300 ± 120 600 ± 45 190 ± 12 16 ± 1.2 22 ± 0.9 28 ± 1.5 539.9 ± 10 140 ± 2.7 44.6 ± 1.9 33.74 ± 2.8 6.36 ± 0.7 2.03 ± 0.1