Influence of in vitro supplementation with lipids from conventional and Alpine milk on fatty acid distribution and cell growth of HT-29 cells

Degen, Christian; Lochner, Alfred; Keller, Sylvia; Kuhnt, Katrin; Dänicke, Sven; Jahreis, Gerhard

doi:10.1186/1476-511X-10-131

Lipids in Health and Disease

Table 1 Fatty acid distribution of milk lipids

From: Influence of in vitro supplementation with lipids from conventional and Alpine milk on fatty acid distribution and cell growth of HT-29 cells

	A								B
	ML_con				ML_alp				ML_con				ML_alp
FAME [%]	Mean		SD		Mean		SD		Mean		SD		Mean		SD
C14:0	12.32	±	0.51	^b	9.67	±	0.25	^d	13.09	±	0.18	^a	10.21	±	0.18	^c
C14:1 c 9	1.04	±	0.05	^b	0.89	±	0.03	^d	1.15	±	0.02	^a	0.98	±	0.01	^c
C15:0	1.24	±	0.05	^d	1.35	±	0.02	^c	1.47	±	0.04	^b	1.58	±	0.04	^a
C16:0	38.36	±	1.29	^b	26.49	±	0.89	^d	43.50	±	1.35	^a	29.76	±	0.53	^c
C16:1 c 9	1.70	±	0.05	^b	1.37	±	0.04	^d	1.97	±	0.01	^a	1.62	±	0.04	^c
C17:0	0.56	±	0.02	^c	0.68	±	0.01	^b	0.68	±	0.02	^b	0.79	±	0.01	^a
C18:0	9.53	±	1.07		10.19	±	0.85		9.92	±	0.22		10.54	±	0.26
C18:1 c 9	14.06	±	0.65	^d	19.79	±	0.68	^b	15.56	±	0.19	^c	21.71	±	0.27	^a
C18:1 c 11	0.46	±	0.02	^d	0.60	±	0.02	^b	0.50	±	0.02	^c	0.66	±	0.02	^a
C18:1 c 12-15	0.40	±	0.02	^b	0.55	±	0.03	^a	0.41	±	0.03	^b	0.56	±	0.05	^a
C18:1 t 4-8	0.09	±	0.01	^b	0.25	±	0.02	^a	0.09	±	0.02	^b	0.25	±	0.01	^b
C18:1 t 9	0.16	±	0.01	^b	0.29	±	0.01	^a	0.15	±	0.03	^b	0.30	±	0.01	^a
C18:1 t 10	0.21	±	0.02	^c	0.28	±	0.02	^b	0.22	±	0.02	^c	0.32	±	0.01	^a
C18:1 t 11 (VA)	0.47	±	0.02	^c	4.54	±	0.13	^b	0.51	±	0.01	^c	4.96	±	0.05	^a
C18:1 t 12-16	0.81	±	0.07	^c	1.25	±	0.10	^b	0.91	±	0.03	^c	1.44	±	0.04	^a
C18:2 n-6	1.06	±	0.09	^b	1.30	±	0.06	^ab	1.48	±	0.43	^ab	1.73	±	0.38	^a
C18:2 n-6 (t,t)	0.30	±	0.02	^c	0.52	±	0.04	^b	0.35	±	0.03	^c	0.71	±	0.06	^a
C18:2 c 9,t 11	0.31	±	0.03	^c	2.77	±	0.13	^b	0.32	±	0.04	^c	3.04	±	0.08	^a
C18:2 t 11,c 13	0.00	±	0.00	^c	0.25	±	0.01	^b	0.00	±	0.00	^c	0.29	±	0.02	^a
C18:2 t 9,t 11	0.03	±	0.01	^c	0.12	±	0.00	^b	0.01	±	0.01	^d	0.16	±	0.02	^a
C18:2 t 11,t 13	0.00	±	0.00	^c	0.05	±	0.00	^a	0.00	±	0.00	^c	0.04	±	0.01	^b
C18:3 n-3	0.34	±	0.03	^d	1.27	±	0.06	^b	0.43	±	0.03	^c	1.46	±	0.02	^a
C20:0	0.21	±	0.11		0.17	±	0.02		0.20	±	0.04		0.19	±	0.03
C20:1 c 9	0.02	±	0.00		0.03	±	0.01		0.04	±	0.02		0.03	±	0.02
C20:3 n-6	0.09	±	0.01		0.07	±	0.01		0.09	±	0.03		0.07	±	0.02
C20:4 n-6	0.10	±	0.01	^a	0.07	±	0.00	^b	0.10	±	0.01	^a	0.08	±	0.02	^b
C20:5 n-3	0.04	±	0.01	^b	0.11	±	0.01	^a	0.03	±	0.02	^b	0.10	±	0.02	^a
C22:5 n-3	0.09	±	0.01	^b	0.12	±	0.00	^ab	0.08	±	0.07	^b	0.16	±	0.05	^a
∑ SCFA(C4 > C8)	6.58	±	0.50	^a	6.56	±	0.40	^a	0.00	±	0.00	^b	0.00	±	0.00	^b
∑ MCFA(C10 > C14)	20.77	±	0.98	^a	16.15	±	0.43	^c	18.50	±	0.95	^b	14.19	±	0.45	^d
∑ SFA	77.73	±	1.00	^a	62.95	±	1.17	^c	75.25	±	0.74	^b	58.98	±	0.35	^d
∑ MUFA	19.89	±	0.81	^d	30.29	±	0.93	^b	21.67	±	0.21	^c	33.02	±	0.33	^a
∑ PUFA incl. CLA	2.38	±	0.19	^d	6.76	±	0.25	^b	3.07	±	0.58	^c	8.01	±	0.38	^a
∑ PUFA n-3	0.48	±	0.05	^c	1.58	±	0.06	^b	0.56	±	0.11	^c	1.83	±	0.05	^a
∑ PUFA n-6	1.26	±	0.10	^b	1.46	±	0.06	^ab	1.83	±	0.48	^a	1.92	±	0.38	^a
PUFA/SFA	0.03	±	0.00	^d	0.11	±	0.01	^b	0.04	±	0.01	^c	0.14	±	0.01	^a
n-6/n-3	2.60	±	0.12	^b	0.93	±	0.01	^c	3.26	±	0.23	^a	1.05	±	0.19	^c
∑ SFA+t FA	79.78	±	0.86	^a	70.08	±	0.90	^c	77.49	±	0.75	^b	66.96	±	0.30	^d
∑ C18:1 (t)	1.74	±	0.13	^c	6.61	±	0.26	^b	1.88	±	0.09	^c	7.28	±	0.07	^a
C18:1 t 9/t 11	0.35	±	0.01	^a	0.06	±	0.00	^c	0.30	±	0.05	^b	0.06	±	0.00	^c
∑ CLA	0.34	±	0.03	^c	3.19	±	0.13	^b	0.33	±	0.04	^c	3.55	±	0.09	^a
VA + C18:2 c 9,t 11	0.78	±	0.04	^c	7.31	±	0.26	^b	0.83	±	0.04	^c	8.00	±	0.13	^a
∑ BCFA	1.75	±	0.05	^d	2.39	±	0.08	^b	2.11	±	0.06	^c	2.80	±	0.03	^a

FA distribution of milk lipids from TAG (ML conventional and ML Alpine) before preparation (A) to FFA-mixtures suitable for cellular experiments, FA distribution of milk lipids as FFA-mixtures used for incubation of cells after saponification, extraction and solution (B) of milk lipids (FFA) in ethanol. Means (n = 6) of FAME [%] significantly differ without the same letter ^{(a, b, c, d)}, two-way ANOVA Tukey post hoc test P < 0.05.

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