Influence of in vitro supplementation with lipids from conventional and Alpine milk on fatty acid distribution and cell growth of HT-29 cells

Degen, Christian; Lochner, Alfred; Keller, Sylvia; Kuhnt, Katrin; Dänicke, Sven; Jahreis, Gerhard

doi:10.1186/1476-511X-10-131

Lipids in Health and Disease

Table 3 Fatty acid distribution of cellular lipids treated with different milk lipids at 8 h

From: Influence of in vitro supplementation with lipids from conventional and Alpine milk on fatty acid distribution and cell growth of HT-29 cells

	Control				ML_con								ML_alp
	0.25% EtOH				100 μM				200 μM				100 μM				200 μM
FAME [%]	Mean		SD		Mean		SD		Mean		SD		Mean		SD		Mean		SD
C14:0	1.58	±	0.39	^d	6.38	±	0.87	^bc	7.96	±	0.94	^a	5.38	±	0.57	^c	6.81	±	0.51	^ab
C14:1 c 9	0.10	±	0.04	^d	0.24	±	0.05	^bc	0.33	±	0.05	^a	0.22	±	0.05	^c	0.30	±	0.03	^ab
C15:0	0.25	±	0.03	^d	1.02	±	0.09	^bc	1.18	±	0.08	^a	1.08	±	0.09	^c	1.28	±	0.07	^ab
C16:0	20.92	±	0.95	^d	34.53	±	1.89	^b	36.96	±	1.35	^a	26.52	±	1.14	^c	26.77	±	0.99	^c
C16:1 c 9	12.75	±	0.74	^a	8.11	±	0.80	^b	6.64	±	0.52	^c	7.11	±	0.51	^bc	5.58	±	0.36	^d
C16:2 t 7,c 9	-				-				-				0.03	±	0.01	^b	0.05	±	0.01	^a
C17:0	0.44	±	0.03	^c	0.64	±	0.04	^b	0.63	±	0.02	^b	0.69	±	0.04	^a	0.67	±	0.02	^ab
C18:0	13.02	±	0.47	^a	10.23	±	0.29	^b	9.34	±	0.63	^c	10.13	±	0.37	^b	8.66	±	0.27	^c
C18:1 c 9	34.32	±	0.82	^a	25.93	±	1.71	^c	25.02	±	1.66	^c	29.23	±	1.32	^b	29.05	±	1.34	^b
C18:1 c 11	3.25	±	0.18	^a	1.85	±	0.24	^b	1.47	±	0.08	^c	1.90	±	0.12	^b	1.56	±	0.07	^c
C18:1 c 12-15	-				0.31	±	0.10	^b	0.39	±	0.09	^ab	0.42	±	0.13	^ab	0.48	±	0.11	^a
C18:1 t 4-8	-				0.03	±	0.01	^bc	0.03	±	0.01	^bc	0.08	±	0.06	^ab	0.11	±	0.05	^a
C18:1 t 9	-				0.10	±	0.03	^b	0.10	±	0.04	^b	0.16	±	0.04	^ab	0.19	±	0.07	^a
C18:1 t 10	-				0.14	±	0.04	^b	0.18	±	0.03	^b	0.19	±	0.04	^b	0.25	±	0.05	^a
C18:1 t 11 (VA)	-				0.27	±	0.03	^c	0.34	±	0.03	^c	2.50	±	0.20	^b	3.32	±	0.16	^a
C18:1 t 12-16	-				0.42	±	0.14	^c	0.54	±	0.18	^bc	0.75	±	0.19	^ab	0.92	±	0.20	^a
C18:2 n-6	2.65	±	0.13	^a	2.08	±	0.05	^cd	2.04	±	0.17	^d	2.28	±	0.07	^b	2.25	±	0.11	^bc
C18:2 n-6 (t,t)	-				0.21	±	0.06	^d	0.29	±	0.03	^c	0.43	±	0.04	^b	0.54	±	0.03	^a
C18:2 c 9,t 11	0.09	±	0.01	^e	0.32	±	0.01	^d	0.37	±	0.03	^c	2.47	±	0.07	^b	3.18	±	0.14	^a
C18:2 t 11,c 13	-				-				-				0.18	±	0.02	^b	0.26	±	0.02	^a
C18:2 t 9,t 11	-				0.03	±	0.01	^cd	0.04	±	0.01	^c	0.18	±	0.02	^b	0.24	±	0.03	^a
C18:3 n-3	0.22	±	0.03	^d	0.33	±	0.02	^c	0.42	±	0.05	^c	0.96	±	0.02	^b	1.31	±	0.09	^a
C20:0	0.38	±	0.03	^a	0.30	±	0.02	^b	0.24	±	0.03	^c	0.28	±	0.02	^b	0.20	±	0.02	^c
C20:1 c 9	0.33	±	0.07	^a	0.17	±	0.04	^bc	0.15	±	0.02	^d	0.23	±	0.04	^b	0.18	±	0.02	^bc
C20:3 n-6	1.19	±	0.14	^a	0.63	±	0.05	^b	0.49	±	0.04	^cd	0.58	±	0.06	^bc	0.42	±	0.05	^d
C20:4 n-6	2.38	±	0.25	^a	1.20	±	0.09	^b	0.86	±	0.05	^c	1.13	±	0.13	^b	0.77	±	0.09	^c
C20:5 n-3	0.70	±	0.05	^a	0.35	±	0.04	^bc	0.27	±	0.02	^d	0.39	±	0.03	^b	0.31	±	0.03	^cd
C22:5 n-3	1.34	±	0.15	^a	0.70	±	0.11	^b	0.53	±	0.06	^c	0.69	±	0.08	^bc	0.54	±	0.07	^bc
C22:6 n-3	1.76	±	0.16	^a	0.82	±	0.11	^b	0.58	±	0.05	^c	0.78	±	0.10	^b	0.53	±	0.07	^c
∑ SFA	38.65	±	0.88	^a	55.47	±	2.59	^b	58.67	±	1.83	^a	46.78	±	1.59	^c	47.29	±	1.66	^c
∑ MUFA	50.84	±	0.34	^b	37.69	±	2.28	^c	35.30	±	1.49	^c	42.91	±	1.34	^b	42.07	±	1.22	^b
∑ PUFA incl. CLA	10.51	±	0.86	^a	6.84	±	0.47	^b	6.03	±	0.44	^b	10.31	±	0.44	^a	10.64	±	0.56	^a
∑ PUFA n-3	4.14	±	0.36	^a	2.31	±	0.28	^c	1.88	±	0.16	^c	2.95	±	0.21	^b	2.82	±	0.22	^b
∑ PUFA n-6	6.28	±	0.51	^a	3.98	±	0.18	^bc	3.46	±	0.25	^d	4.07	±	0.25	^b	3.54	±	0.23	^cd
PUFA/SFA	0.27	±	0.03	^a	0.12	±	0.01	^c	0.10	±	0.01	^c	0.22	±	0.02	^b	0.23	±	0.02	^b
n-6/n-3	1.52	±	0.06	^b	1.74	±	0.17	^a	1.85	±	0.07	^a	1.38	±	0.03	^bc	1.26	±	0.02	^c
∑ SFA + t FA	38.65	±	0.88	^d	56.71	±	2.73	^b	60.23	±	2.06	^a	50.97	±	2.04	^c	52.73	±	1.91	^c
∑ C18:1 (t)	-				0.96	±	0.18	^c	1.18	±	0.26	^c	3.68	±	0.46	^b	4.79	±	0.38	^a
C18:1 t 9,t 11	-				0.37	±	0.10	^a	0.28	±	0.09	^a	0.06	±	0.01	^b	0.06	±	0.02	^b
∑ CLA	0.09	±	0.01	^d	0.35	±	0.02	^c	0.41	±	0.04	^c	2.83	±	0.07	^b	3.68	±	0.15	^a
VA + C18:2 c 9,t 11	0.09	±	0.01	^d	0.59	±	0.04	^c	0.71	±	0.03	^c	4.97	±	0.25	^b	6.50	±	0.30	^a
∑ BCFA	0.44	±	0.12	^d	1.59	±	0.15	^c	1.83	±	0.13	^b	1.97	±	0.16	^b	2.38	±	0.14	^a

FA distribution of HT-29 cells after incubation with milk lipids (ML conventional and ML Alpine) given as FFA-mixtures in ethanol. Means (n = 6) of FAME [%] significantly differ without the same letter ^{(a, b, c, d)}, two-way ANOVA Tukey post hoc test P < 0.05.

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