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Table 5 CLA isomers in cellular lipids of HT-29 treated with different milk lipids at 8 h

From: Influence of in vitro supplementation with lipids from conventional and Alpine milk on fatty acid distribution and cell growth of HT-29 cells

 

Control

 

MLcon

MLalp

 

0.25% EtOH

 

100 μM

 

200 μM

 

100 μM

 

200 μM

 

CLA FAME [%]

Mean

 

SD

 

Mean

 

SD

 

Mean

 

SD

 

Mean

 

SD

 

Mean

 

SD

 

t 12,t 14

-

 

-

 

-

 

-

 

-

 

t 11,t 13

-

 

2.63

±

0.71

a

2.76

±

0.26

a

1.26

±

0.14

b

1.31

±

0.06

b

t 10,t 12

-

 

0.69

±

0.23

a

0.82

±

0.09

a

0.24

±

0.09

b

0.26

±

0.09

b

t 9,t 11

3.15

±

1.28

a

3.08

±

0.49

a

3.35

±

0.51

a

1.18

±

0.35

b

1.14

±

0.34

b

t 8,t 10

-

 

0.28

±

0.15

ab

0.47

±

0.16

a

0.02

±

0.01

bc

0.11

±

0.02

bc

t 7,t 9

2.86

±

0.79

a

0.89

±

0.28

b

0.73

±

0.08

b

0.09

±

0.05

b

0.05

±

0.01

b

∑ trans, trans

6.02

±

2.06

bc

8.38

±

1.19

ab

9.68

±

0.30

a

3.48

±

0.53

c

3.57

±

0.50

c

t 11,c 13

4.88

±

3.15

ab

3.59

±

1.20

ab

1.81

±

0.30

b

6.54

±

0.47

a

6.83

±

0.34

a

c 11,t 13

3.00

±

1.89

a

1.80

±

0.93

ab

1.17

±

0.25

ab

0.31

±

0.06

b

0.36

±

0.10

b

t 10,c 12

3.82

±

1.39

a

3.48

±

1.11

b

1.61

±

0.75

ab

0.42

±

0.27

b

0.22

±

0.15

b

c 9,t 11

77.28

±

3.25

b

74.65

±

0.80

a

76.12

±

0.98

b

86.66

±

1.17

a

87.14

±

0.80

a

t 8,c 10

0.63

±

0.45

ab

0.78

±

0.58

ab

1.20

±

0.25

a

0.44

±

0.10

ab

0.18

±

0.10

b

t 7,c 9

4.38

±

1.55

bc

6.09

±

1.58

cd

7.18

±

0.55

a

1.82

±

0.20

cd

1.44

±

0.11

d

∑ cis,trans/trans,cis

93.98

±

2.06

a

90.39

±

1.23

b

89.09

±

0.38

b

96.19

±

0.61

a

96.17

±

0.59

a

c 11,c 13

-

 

-

 

-

 

0.08

±

0.02

 

0.03

±

0.00

 

c 10,c 12

-

 

-

 

-

 

-

 

-

 

c 9,c 11

-

 

1.23

±

0.10

a

1.18

±

0.09

a

0.26

±

0.08

b

0.23

±

0.09

b

c 8,c 10

-

 

-

 

-

 

-

 

-

 

∑ cis/cis

-

 

1.23

±

0.10

a

1.18

±

0.09

a

0.34

±

0.09

b

0.26

±

0.09

b

t 11,c 13/t 7,c 9

1.33

±

1.25

b

0.64

±

0.34

b

0.25

±

0.06

b

3.63

±

0.46

a

4.77

±

0.55

a

  1. CLA distribution of HT-29 cells after incubation with milk lipids (ML conventional and ML Alpine) given as FFA-mixtures in ethanol. Means (n = 6) of FAME [%] significantly differ without the same letter (a, b, c, d), two-way ANOVA Tukey post hoc test P < 0.05.