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Table 5 CLA isomers in cellular lipids of HT-29 treated with different milk lipids at 8 h

From: Influence of in vitro supplementation with lipids from conventional and Alpine milk on fatty acid distribution and cell growth of HT-29 cells

  Control   MLcon MLalp
  0.25% EtOH   100 μM   200 μM   100 μM   200 μM  
CLA FAME [%] Mean   SD   Mean   SD   Mean   SD   Mean   SD   Mean   SD  
t 12,t 14 -   -   -   -   -  
t 11,t 13 -   2.63 ± 0.71 a 2.76 ± 0.26 a 1.26 ± 0.14 b 1.31 ± 0.06 b
t 10,t 12 -   0.69 ± 0.23 a 0.82 ± 0.09 a 0.24 ± 0.09 b 0.26 ± 0.09 b
t 9,t 11 3.15 ± 1.28 a 3.08 ± 0.49 a 3.35 ± 0.51 a 1.18 ± 0.35 b 1.14 ± 0.34 b
t 8,t 10 -   0.28 ± 0.15 ab 0.47 ± 0.16 a 0.02 ± 0.01 bc 0.11 ± 0.02 bc
t 7,t 9 2.86 ± 0.79 a 0.89 ± 0.28 b 0.73 ± 0.08 b 0.09 ± 0.05 b 0.05 ± 0.01 b
trans, trans 6.02 ± 2.06 bc 8.38 ± 1.19 ab 9.68 ± 0.30 a 3.48 ± 0.53 c 3.57 ± 0.50 c
t 11,c 13 4.88 ± 3.15 ab 3.59 ± 1.20 ab 1.81 ± 0.30 b 6.54 ± 0.47 a 6.83 ± 0.34 a
c 11,t 13 3.00 ± 1.89 a 1.80 ± 0.93 ab 1.17 ± 0.25 ab 0.31 ± 0.06 b 0.36 ± 0.10 b
t 10,c 12 3.82 ± 1.39 a 3.48 ± 1.11 b 1.61 ± 0.75 ab 0.42 ± 0.27 b 0.22 ± 0.15 b
c 9,t 11 77.28 ± 3.25 b 74.65 ± 0.80 a 76.12 ± 0.98 b 86.66 ± 1.17 a 87.14 ± 0.80 a
t 8,c 10 0.63 ± 0.45 ab 0.78 ± 0.58 ab 1.20 ± 0.25 a 0.44 ± 0.10 ab 0.18 ± 0.10 b
t 7,c 9 4.38 ± 1.55 bc 6.09 ± 1.58 cd 7.18 ± 0.55 a 1.82 ± 0.20 cd 1.44 ± 0.11 d
cis,trans/trans,cis 93.98 ± 2.06 a 90.39 ± 1.23 b 89.09 ± 0.38 b 96.19 ± 0.61 a 96.17 ± 0.59 a
c 11,c 13 -   -   -   0.08 ± 0.02   0.03 ± 0.00  
c 10,c 12 -   -   -   -   -  
c 9,c 11 -   1.23 ± 0.10 a 1.18 ± 0.09 a 0.26 ± 0.08 b 0.23 ± 0.09 b
c 8,c 10 -   -   -   -   -  
cis/cis -   1.23 ± 0.10 a 1.18 ± 0.09 a 0.34 ± 0.09 b 0.26 ± 0.09 b
t 11,c 13/t 7,c 9 1.33 ± 1.25 b 0.64 ± 0.34 b 0.25 ± 0.06 b 3.63 ± 0.46 a 4.77 ± 0.55 a
  1. CLA distribution of HT-29 cells after incubation with milk lipids (ML conventional and ML Alpine) given as FFA-mixtures in ethanol. Means (n = 6) of FAME [%] significantly differ without the same letter (a, b, c, d), two-way ANOVA Tukey post hoc test P < 0.05.