Accumulation and aberrant composition of cholesteryl esters in Scrapie-infected N2a cells and C57BL/6 mouse brains

Table 1 Amplex Red vs. HPLC-MS cholesterol composition in N2a and ScN2a cells.

Method	Cell lines	nmol/10⁶ cells			CE/TC %
		TC	FC	CE
Amplex Red	N2a	80.11 ± 4.18	77.52 ± 0.38	2.59 ± 1.92	3.23
	ScN2a	83.60 ± 4.18	79.80 ± 0.88*	3.81 ± 4.08	4.55
		p = 0.364	*p = 0.015*	p = 0.664
HPLC-MS	N2a	135.36 ± 13.59	98.38 ± 9.75	36.98 ± 4.01	27.0
	ScN2a	200.37 ± 8.27*	146.54 ± 4.76*	53.83 ± 3.60*	26.8
		*p = 0.002*	*p = 0.002*	*p = 0.006*

Total (TC), free (FC) and esterified (CE) cholesterol in uninfected (N2a) and persistently prion-infected (ScN2a) cell extracts. Values represent the mean ± SD of triplicate determinations and are representative of at least three independent trials. *Statistical significance by unpaired Student's t-test.

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