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Table 1 Amplex Red vs. HPLC-MS cholesterol composition in N2a and ScN2a cells.

From: Accumulation and aberrant composition of cholesteryl esters in Scrapie-infected N2a cells and C57BL/6 mouse brains

Method Cell lines nmol/106 cells CE/TC %
   TC FC CE  
Amplex Red N2a 80.11 ± 4.18 77.52 ± 0.38 2.59 ± 1.92 3.23
  ScN2a 83.60 ± 4.18 79.80 ± 0.88* 3.81 ± 4.08 4.55
   p = 0.364 p = 0.015 p = 0.664  
HPLC-MS N2a 135.36 ± 13.59 98.38 ± 9.75 36.98 ± 4.01 27.0
  ScN2a 200.37 ± 8.27* 146.54 ± 4.76* 53.83 ± 3.60* 26.8
   p = 0.002 p = 0.002 p = 0.006  
  1. Total (TC), free (FC) and esterified (CE) cholesterol in uninfected (N2a) and persistently prion-infected (ScN2a) cell extracts. Values represent the mean ± SD of triplicate determinations and are representative of at least three independent trials. *Statistical significance by unpaired Student's t-test.