Differences in apolipoprotein mRNA and nuclear receptor protein levels among M-Hep and S-Hep. (A) Analyses of mRNA levels of apoE and apoA-I with quantitative RT-PCR (n = 4). GAPDH mRNA was used as the endogenous control for the expression levels of the gene of interest. (B, C) Western blot analyses were performed with the proteins prepared from nuclear fractions (Panel B), and nuclear protein levels were quantified with Image J utilizing lamin A/C as the internal control (Panel C) (n = 3). Y-axes in panels A and C represent ratios to the data of M-Hep.