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Figure 1 | Lipids in Health and Disease

Figure 1

From: Apolipoprotein A1/C3/A5 haplotypes and serum lipid levels

Figure 1

Genotyping of the ApoA1/C3/A5 gene polymorphisms. (A) ApoA1 -75 bp G>A. Lane M, 50 bp marker ladder; lanes 1 and 2, the PCR products of the samples (259 bp); lanes 3 and 4, GA genotype (254-, 176- and 78-bp); lanes 5 and 6, GG genotype (176- and 78-bp); and lanes 7 and 8, AA genotype (254 bp). (B) ApoC3 3238C>G. Lane M, 100 bp marker ladder; lanes 1 and 2, the PCR products of the samples (596 bp); lanes 3 and 4, CC genotype (596 bp); lanes 5 and 6, CG genotype (596-, 371- and 225-bp); and lanes 7 and 8, GG genotype (371- and 225-bp). (C) ApoA5 -1131T>C. Lanes 1 and 2, the PCR products of the samples (188 bp); lanes 3 and 4, TT genotype (165- and 23-bp); lanes 5 and 6, TC genotype (188-, 165- and 23-bp); and Lanes 7 and 8, CC genotype (188 bp). The 23 bp fragment was invisible in the gel owing to its fast migration speed. (D) ApoA5 c.553G>T. Lane M, 50 bp marker ladder; lanes 1 and 2, the PCR products of the samples (211 bp); lanes 3 and 4, GG genotype (176- and 35-bp); lanes 5 and 6, GT genotype (211-, 176- and 35-bp). TT genotype was not detected in both sexes. The 35 bp fragment was invisible in the gel owing to its fast migration speed. (E) ApoA5 c.457G>A. Lanes 1 and 2, the PCR products of the samples (211 bp); lanes 3 and 4, GG genotype (211 bp); lanes 5 and 6, GA genotype (211-, 133- and 78-bp); and lanes 7 and 8, AA genotype (133- and 78-bp).

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