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Table 1 Chronic Stress Plan Table

From: The model of rat lipid metabolism disorder induced by chronic stress accompanying high-fat-diet

D36
Fasting
D37
Fasting
D38
Water Deprivation
D39
Night Lighting
D40
Day-Night Inversion
D41
Wet Feeding
D42
Water Deprivation
D43
Day-Night Inversion
D44
Vibration
D45
Pinpricking
D46
Clamping the Tail
D47
Vibration
D48
Swimming in Iced Water
D49
Day-Night Inversion
D50
Noise
D51
Fasting
D52
Fasting
D53
Pinpricking
D54
Swimming in Iced Water
D55
Noise
D56
Clamping the Tail
  1. Fasting: Not feeding in 48 hours, free access to water, twice; Water Deprivation: Not watering in 24 hours, free access to food, twice; Day-Night Inversion: covering the cages with lightproof but venting cloth and lighting the cages all the night, twice; Night Lighting: Keeping lighting the whole day, Twice; Swimming in Iced Water: putting ice in cold water, making the temperature 4 degrees Celsius, and then putting the wistar rats in the water for 5 minutes, twice; Clamping the Tail: Clamping the tails of the wistar rats until they struggle and scream, twice; Vibration: Vibrating the cage in order to make the wistar rats can not stand steadily, twice; Pinpricking: using the syringe needle to prink the body of the wistar rats, twice; Noise: Using iron lids to hit the cage until the wistar rats spook or squeeze, twice; Wet Feeding: Wetting the wooden meals and making the wistar rats live in them for 24 hour, once.