Figure 3
From: Enchanced levels of apolipoprotein M during HBV infection feedback suppresses HBV replication
Effects of HBV on ApoM expression in transfected cells. (A) Effect of HBV on ApoM promoter activity. HepG2 cells were co-transfected with pGL3-ApoM-Luc and pBlueks-HBV1.3. PBlueks was used as vector control. Luciferase activities were determined and analyzed. (B) Effects of HBV on ApoM mRNA expression. HepG2 cells were transfected with pBlueks-HBV1.3 or pBlueks. ApoM mRNA was determined by semi-quantitative RT-PCR using primers specific to each of the genes, respectively. (D) Effects of HBV on ApoM protein expression. HepG2 cells were transfected with pBlueks-HBV1.3 or pBlueks. The levels of ApoM protein were determined by ELISA.