Figure 15
From: Effect of polyunsaturated fatty acids on drug-sensitive and resistant tumor cells in vitro
Effect of anti-oxidants vitamin E and SOD (superoxide dismutase) on the generation of free radicals (superoxide anion) and lipid peroxides induced by GLA, EPA and DHA in KB-3-1 cells in vitro on day 3 of incubation with fatty acids. Superoxide anion generated in KB-3-1 cells was measured as NBT reduction (expressed as μ moles of diformazan formed) and lipid peroxides formed is measured by TBA reaction and expressed as pmoles of MDA-eq formed. All studies were done on day 3 following incubation with 40 μg/ml of fatty acids with or without vitamin E or SOD. All values are Mean ± SD. * P < 0.05 compared to control; #P < 0.05 compared to fatty acid treatment.