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Table 2 Antibacterial and antifungal activity of the essential oil of M. vulgare using agar disc diffusion, IC50 and minimal inhibition concentration (MIC).

From: The in-vitro evaluation of antibacterial, antifungal and cytotoxic properties of Marrubium vulgare L. essential oil grown in Tunisia

Strains DDa IC50b MICc DDd
Bacterial strains Gram (+)     
Staphylococcus aureus 12.0 ± 0.5 2500 ± 50 >1100.00 20 ± 0.5
Staphylococcus epidermidis 25.2 ± 0.3 2200 ± 50 >590.00 26 ± 0.5
Micrococcus luteus 12.0 ± 0.5 1120 ± 50 >560.00 20 ± 1.5
Enterococcus faecalis 9.6 ± 1.0 1140 ± 10 >680.00 25 ± 1.0
Enterobacter cloacae 13.8 ± 0.6 1500 ± 90 >670.00 21 ± 1.4
Staphylococcus aureus 25923 18 ± 1.0 2600 ± 80 >1150.00 24 ± 0.5
Bacillus subtilis 13.2 ± 1.0 2130 ± 50 >890.00 26 ± 0.6
Bacillus cereus 6.6 ± 0.4 2113 ± 40 >950.00 21 ± 1.0
Bacterial strains Gram (-)     
Botrytis cinerea NS NS NS 21 ± 0.5
Pseudomonas aeruginosa 27853 NS NS NS 20 ± 1.0
Klebsielle pneumoniae WHO24 NS NS NS 21 ± 0.9
Escherchia coli 25922 NS NS NS 22 ± 0.8
Salmonella NS NS NS 29 ± 1.0
Fungal strains     
Botrytis cinerea 12.6 ± 0.5 2190 ± 12 >1100.00 29 ± 1.0
Fusarium solani 6.9 ± 0.5 3220 ± 20 >1190.00 28 ± 0.6
Penicillium digitatum 6.6 ± 0.4 3200 ± 15 >1120.00 21 ± 0.9
Aspergillus. niger 6.4 ± 0.0 3000 ± 50 >1180.00 30 ± 0.5
  1. Results are means of three different experiments,
  2. a DD: Disc Diameter of inhibition (halo size) in (mm), E. oil 100 μg/disc,
  3. b MIC: minimum inhibitory concentration (μg/ml),
  4. cIC50: 50% inhibition concentration (μg/ml),
  5. dDD: Disc Diameter of inhibition zone of ampicillin (10 μg/disc) and cycloheximide (10 μg/disc), were used as positive controls for bacteria and fungi, respectively,
  6. NS: not sensitive.