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Table 2 Antibacterial and antifungal activity of the essential oil of M. vulgare using agar disc diffusion, IC50 and minimal inhibition concentration (MIC).

From: The in-vitro evaluation of antibacterial, antifungal and cytotoxic properties of Marrubium vulgare L. essential oil grown in Tunisia

Strains

DDa

IC50b

MICc

DDd

Bacterial strains Gram (+)

    

Staphylococcus aureus

12.0 ± 0.5

2500 ± 50

>1100.00

20 ± 0.5

Staphylococcus epidermidis

25.2 ± 0.3

2200 ± 50

>590.00

26 ± 0.5

Micrococcus luteus

12.0 ± 0.5

1120 ± 50

>560.00

20 ± 1.5

Enterococcus faecalis

9.6 ± 1.0

1140 ± 10

>680.00

25 ± 1.0

Enterobacter cloacae

13.8 ± 0.6

1500 ± 90

>670.00

21 ± 1.4

Staphylococcus aureus 25923

18 ± 1.0

2600 ± 80

>1150.00

24 ± 0.5

Bacillus subtilis

13.2 ± 1.0

2130 ± 50

>890.00

26 ± 0.6

Bacillus cereus

6.6 ± 0.4

2113 ± 40

>950.00

21 ± 1.0

Bacterial strains Gram (-)

    

Botrytis cinerea

NS

NS

NS

21 ± 0.5

Pseudomonas aeruginosa 27853

NS

NS

NS

20 ± 1.0

Klebsielle pneumoniae WHO24

NS

NS

NS

21 ± 0.9

Escherchia coli 25922

NS

NS

NS

22 ± 0.8

Salmonella

NS

NS

NS

29 ± 1.0

Fungal strains

    

Botrytis cinerea

12.6 ± 0.5

2190 ± 12

>1100.00

29 ± 1.0

Fusarium solani

6.9 ± 0.5

3220 ± 20

>1190.00

28 ± 0.6

Penicillium digitatum

6.6 ± 0.4

3200 ± 15

>1120.00

21 ± 0.9

Aspergillus. niger

6.4 ± 0.0

3000 ± 50

>1180.00

30 ± 0.5

  1. Results are means of three different experiments,
  2. a DD: Disc Diameter of inhibition (halo size) in (mm), E. oil 100 μg/disc,
  3. b MIC: minimum inhibitory concentration (μg/ml),
  4. cIC50: 50% inhibition concentration (μg/ml),
  5. dDD: Disc Diameter of inhibition zone of ampicillin (10 μg/disc) and cycloheximide (10 μg/disc), were used as positive controls for bacteria and fungi, respectively,
  6. NS: not sensitive.