7-ketocholesterol content in control and glycolaldehyde-treated macrophages. J774 macrophages were overloaded with oxidized LDL (50 μg/mL de DMEM) for 48 h. In the last 5h, cells were incubated with 0.25 mM glycolaldehyde (GAD; black bars) and 1 μM LXR agonist (T0901317). Control cells (C; gray bars) were incubated with T0901317 alone. After washing, macrophages were incubated for 5 h with HDL alone (50 μg/mL de DMEM) or HDL + T0901317. 7-ketocholesterol was determined after lipid cell extraction by CG/MS, utilizing 5-alpha cholestane as an internal standard.