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Figure 3 | Lipids in Health and Disease

Figure 3

From: Advanced Glycation in macrophages induces intracellular accumulation of 7-ketocholesterol and total sterols by decreasing the expression of ABCA-1 and ABCG-1

Figure 3

7-ketocholesterol content in control and glycolaldehyde-treated macrophages. J774 macrophages were overloaded with oxidized LDL (50 μg/mL de DMEM) for 48 h. In the last 5h, cells were incubated with 0.25 mM glycolaldehyde (GAD; black bars) and 1 μM LXR agonist (T0901317). Control cells (C; gray bars) were incubated with T0901317 alone. After washing, macrophages were incubated for 5 h with HDL alone (50 μg/mL de DMEM) or HDL + T0901317. 7-ketocholesterol was determined after lipid cell extraction by CG/MS, utilizing 5-alpha cholestane as an internal standard.

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Lipids in Health and Disease

ISSN: 1476-511X