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Table 2 Oxysterols and total cholesterol in control (C) and glycolaldehyde-treated macrophages (GAD)

From: Advanced Glycation in macrophages induces intracellular accumulation of 7-ketocholesterol and total sterols by decreasing the expression of ABCA-1 and ABCG-1

Macrophage   C    GAD  
  - HDL HDL
+
T0901317
- HDL HDL
+
T0901317
7-ketocholesterol 86.7 ± 16 72.8 ± 14* 68.4 ± 14* 83.8 ± 19 74.2 ± 13 79.8 ± 19
7β-hydroxicholesterol 26.7 ± 9 20.4 ± 6 21.5 ± 10 25.5 ± 10 23.6 ± 6 22.8 ± 10
7α-hydroxicholesterol 9.2 ± 4 8.1 ± 3 8.1 ± 5 9.4 ± 3 8.8 ± 2 8.6 ± 4
cholesterol-5,6 β 22.6 ± 9 20 ± 5 16.9 ± 6 24.2 ± 6 22.9 ± 6 20.5 ± 6
cholesterol- 5,6 α 7.1 ± 2 6.6 ± 2 5.5 ± 2 7.4 ± 2 6.2 ± 2 6.7 ± 3
24.25 epoxycholesterol 3.8 ± 2 3.5 ± 2 2.8 ± 1 3.9 ± 2 3.7 ± 2 3.5 ± 2
27-hydroxycholesterol 0.48 ± 0.2 0.51 ± 0.3 0.44 ± 0.2 0.55 ± 0.3 0.52 ± 0.2 0.48 ± 0.2
25-hydroxycholesterol 0.35 ± 0.3 0.38 ± 0.1 0.42 ± 0.2 0.43 ± 0.2 0.37 ± 0.1 0.40 ± 0.2
cholestan-3β,5α,6β-triol 10 ± 3 8.3 ± 2 8.5 ± 5 10.5 ± 4 9.4 ± 3 9.2 ± 3
total cholesterol 244 ± 57 203 ± 47* 191 ± 44* 227 ± 52 214 ± 53 224 ± 50
  1. Values were corrected by 5-alpha cholestane (internal standard) and are expressed as ng/μg of cell protein ± DP (n = 12).
  2. * p < 0.05 comparison were made between cells maintained in the presence of culture medium alone with HDL or HDL + T0901317. One way ANOVA and Bonferroni post test (mean ± SD).