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Table 2 Oxysterols and total cholesterol in control (C) and glycolaldehyde-treated macrophages (GAD)

From: Advanced Glycation in macrophages induces intracellular accumulation of 7-ketocholesterol and total sterols by decreasing the expression of ABCA-1 and ABCG-1

Macrophage

 

C

  

GAD

 
 

-

HDL

HDL

+

T0901317

-

HDL

HDL

+

T0901317

7-ketocholesterol

86.7 ± 16

72.8 ± 14*

68.4 ± 14*

83.8 ± 19

74.2 ± 13

79.8 ± 19

7β-hydroxicholesterol

26.7 ± 9

20.4 ± 6

21.5 ± 10

25.5 ± 10

23.6 ± 6

22.8 ± 10

7α-hydroxicholesterol

9.2 ± 4

8.1 ± 3

8.1 ± 5

9.4 ± 3

8.8 ± 2

8.6 ± 4

cholesterol-5,6 β

22.6 ± 9

20 ± 5

16.9 ± 6

24.2 ± 6

22.9 ± 6

20.5 ± 6

cholesterol- 5,6 α

7.1 ± 2

6.6 ± 2

5.5 ± 2

7.4 ± 2

6.2 ± 2

6.7 ± 3

24.25 epoxycholesterol

3.8 ± 2

3.5 ± 2

2.8 ± 1

3.9 ± 2

3.7 ± 2

3.5 ± 2

27-hydroxycholesterol

0.48 ± 0.2

0.51 ± 0.3

0.44 ± 0.2

0.55 ± 0.3

0.52 ± 0.2

0.48 ± 0.2

25-hydroxycholesterol

0.35 ± 0.3

0.38 ± 0.1

0.42 ± 0.2

0.43 ± 0.2

0.37 ± 0.1

0.40 ± 0.2

cholestan-3β,5α,6β-triol

10 ± 3

8.3 ± 2

8.5 ± 5

10.5 ± 4

9.4 ± 3

9.2 ± 3

total cholesterol

244 ± 57

203 ± 47*

191 ± 44*

227 ± 52

214 ± 53

224 ± 50

  1. Values were corrected by 5-alpha cholestane (internal standard) and are expressed as ng/μg of cell protein ± DP (n = 12).
  2. * p < 0.05 comparison were made between cells maintained in the presence of culture medium alone with HDL or HDL + T0901317. One way ANOVA and Bonferroni post test (mean ± SD).