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Figure 2 | Lipids in Health and Disease

Figure 2

From: Suppression of nitric oxide induction and pro-inflammatory cytokines by novel proteasome inhibitors in various experimental models

Figure 2

Effects of proteasome inhibitors on chymotrypsin-like activity of 20S rabbit muscle proteasomes: 20S Rabbit muscle proteasomes were treated with various compounds with 100 μL dissolved in 0.5% DMSO of dexamethasone (10 μM); mevinolin (20 μM); α-tocopherol (100 μM); δ-tocotrienol (10 μM); riboflavin (40 μM); or quercetin-HCL (40 μM) for 30 min. Then, proteolytic activity was measured by adding succinyl-Leu-Leul-Val-Tyr-amino methyl coumarin as substrate and measuring fluorescence (absorption at 360 nm and emission at 460 nm) using an Flx 800 microplate fluorescence reader. The treatments 1-7 correspond to: 1. Control (tris + 20S proteasomes + 0.5% DMSO); 2. dexamethasone; 3. mevinolin; 4. α-tocopherol; 5. δ-tocotrienol; 6. riboflavin; 7. quercetin-HCL.

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Lipids in Health and Disease

ISSN: 1476-511X