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Figure 5 | Lipids in Health and Disease

Figure 5

From: Suppression of nitric oxide induction and pro-inflammatory cytokines by novel proteasome inhibitors in various experimental models

Figure 5

The effect of proteasome inhibitors on translocation of NF-κB to the nucleus in LPS-stimulated RAW 264.7 cells. RAW 264.7 cells (1 × 106 cells/500 μL/well) for 2 h, after 2 h, the cells were treated with 100 μL dissolved in 0.5% DMSO of dexamethasone (10 μM), mevinolin (20 μM), α-tocopherol (100 μM), δ-tocotrienol (10 μM), riboflavin (40 μM), or quercetin-HCL (40 μM) for 1 h. Then all the wells were challenged with LPS (10 ng/well; 400 μL) and incubated at 37°C 5% CO2 for 36 h. At that time nuclear protein was extracted NF-κB activation was measured using "electronic mobility shift assay" (EMSA). Data are presented as the percent of nuclear NF-κB levels compared to controls. The treatments 1-7 correspond to: 1. Control (media + cells + LPS [10 ng/mL] + 0.5% DMSO); 2. dexamethasone; 3. mevinolin; 4. α-tocopherol; 5. δ-tocotrienol; 6. riboflavin; 7. quercetin-HCL.

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