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Figure 9 | Lipids in Health and Disease

Figure 9

From: Suppression of nitric oxide induction and pro-inflammatory cytokines by novel proteasome inhibitors in various experimental models

Figure 9

Effects of various compounds on the secretion of TNF-α, in LPS-stimulated thioglycolate-elicited peritoneal macrophages of 8-week-old C57BL/6, BALB/c, double subunits knockout (LMP-7/MECL-1-/-), and PPAR-α,-/- female mice. Thioglycolate-elicited peritoneal macrophages of each mouse were adhered to the bottom of 12 well plates (1 × 107 cells/well in 1.0 mL media) for 4 h. After 4 h, the cells were washed with media three times. The cells were cultured overnight in the fresh media (500 μL) at 37°C in an incubator at 5% CO2 after the final wash. After 4 h the cells were treated with each compound (100 μL dissolved in 0.2% DMSO) of α,-tocopherol, 100 μM; δ-tocotrienol, 10 μM; riboflavin, 40 μM; or quercetin-HCL, 40 μM for I h, and the LPS (10 μL of 1.0 μg/mL) was added to assay mixture. The assay mixtures were incubated for 4 h at room temperature. The supernatants were collected after 4 h of LPS challenge, and stored at -70°C to carry out TNF-α assay by ELISA. The cells viability were very good (> 95%) in all the treatments. The treatments 1-5 correspond to: 1. Control (media + macrophages (cells) + LPS + 0.2% DMSO); 2. α-tocopherol; 3. δ-tocotrienol; 4. riboflavin; 5. quercetin-HCL.

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