Suppression of nitric oxide induction and pro-inflammatory cytokines by novel proteasome inhibitors in various experimental models

Table 1 Effects of various compounds on the chymotrypsin-like activity of 20S rabbit muscle proteasomes¹

Dexame = Dexamethasone; Mevino = Mevinolin; α-Tocop = α-Tocopherol; δ-Tocotri = δ-Tocotrienol; Ribofl = Riboflavin; Querce = Quercetin;
Amilor = Amiloride; (-) Cor Lact = (-) Corey Lactone; 20S Prot = 20S Proteasomes.
¹Chymotrypsin-like activity was carried out by using synthetic dipeptide substrate III (Suc-Leu-Leu-Val-Tyr-AMC) in Tris buffer pH 7.5, 0.02 M. 20S rabbit muscle proteasomes was used. Time of incubation was 30 minutes.
²Fluorescence absorption was measured at excitation = 360 nm and emission = 460 nm.
³Each compound was dissolved in dimethyl sulfoxide (0.5% DMSO). Appropriate amount of each compound (highest concentration) was dissolved in 1.0 mL DMSO = A stock solution; 50 μL of stock solution A was mixed with 950 μL of media = B solution. The remaining concentrations were prepared from B solution (1:2 dilution) successively.
⁴Percentage of control values are in parentheses.

ISSN: 1476-511X