Lipids in Health and Disease

Table 5 Real-time PCR primer sequences for genes of interest

From: The effect of short-term canola oil ingestion on oxidative stress in the vasculature of stroke-prone spontaneously hypertensive rats

Gene	Forward primer (5'-3')	Reverse primer (5'-3')
NOX 2	TCA AGT GTC CCC AGG TAT CC	CTT CAC TGGCTGTACCAAAGG
p22^phox	GCT CAT CTG TCT GCT GGA GTA	ACGACCTCATCTGTCACTGGA
CuZn-SOD	TGTGTCCATTGAAGATCGTGTGA	TCTTGTTTCTCGTGGACCACC
Mn-SOD	TTAACGCGCAGATCATGCA	CCTCGGTGACGTTCAGATTGT
Ec-SOD	GGCCCAGCTCCAGACTTGA	CTCAGGTCCCCGAACTCATG

The primers were all obtained from previous published sequences and were ordered through Geneworks (Australia): NOX2 [37], p22^phox [35], CuZn-SOD, MnSOD and Ec-SOD [36].

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ISSN: 1476-511X

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