Effect of dietary n-3 (fish oil) and n-6 PUFA (safflower oil) on fasting plasma lipid and lipoprotein cholesterol concentrations in cholesterol-supplemented (A) and cholesterol-depleted (B) hamsters. Retro-orbital blood was collected into EDTA coated tubes from fasted hamsters. Plasma cholesterol and triglyceride concentrations were determined enzymatically. Bars represent means ± SEM, n = 15-16 animals per group. Appropriate transformations of the data (log HDL; square root total cholesterol, non-HDL cholesterol; inverse triglyceride) were made before statistical analysis. Asterisks indicate significant differences between safflower and fish oil within cholesterol-supplemented (+C) or depleted (-C) hamsters, P ≤ 0.05.