Effect of dietary n-3 (fish oil) and n-6 PUFA (safflower oil) on hepatic mRNA levels of genes associated with cholesterol and lipoprotein metabolism in cholesterol-supplemented (A) and cholesterol-depleted (B) hamsters. Real time PCR was used to measure gene expression in the liver. A standard curve was run on all plates for each mRNA of interest to calculate relative levels. Values were normalized using beta-actin as an endogenous control. Bars represent means ± SEM, n = 14-16 animals per group. Appropriate transformations of the data (log SREBP-2, CYP7A1, MTP, apo B-100, ABCA1, HMG-CoA reductase; square root SR-B1, SREBP-1c, apo A-I) were made before statistical analysis. Asterisks indicate significant differences between safflower oil and fish oil within cholesterol-supplemented (+C) or depleted (-C) hamsters, P ≤ 0.05.