Effect of dietary n-3 (fish oil) and n-6 PUFA (safflower oil) on hepatic protein levels of genes associated with cholesterol and lipoprotein metabolism in cholesterol-supplemented (A) and cholesterol-depleted (B) hamsters. Proteins were extracted from the liver, separated by SDS-PAGE and detected by immunoblotting. Relative protein levels were normalized to the density of beta-actin. Bars represent means ± SEM, n = 14-16 animals per group. Appropriate transformations of the data (log apo A-I, apo E, LDL receptor, SREBP-1c, SR-B1; square root apo B-100) were made before statistical analysis. Asterisks indicate significant differences between safflower oil and fish oil within cholesterol-supplemented (+C) or depleted (-C) hamsters, P ≤ 0.05.