Effect of dietary n-3 (fish oil) and n-6 PUFA (safflower oil) on mRNA levels of small intestinal sterol transporters in cholesterol-supplemented (A) and cholesterol-depleted (B) hamsters. Real time PCR was used to measure gene expression in the small intestine. A standard curve was run on all plates for each mRNA of interest to calculate relative levels. Values were normalized using beta-actin as an endogenous control. Bars represent means ± SEM, n = 14-16 animals per group. Appropriate transformations of the data (log ABCG5) were made before statistical analysis. Asterisks indicate significant differences between safflower oil and fish oil within cholesterol supplemented (+C) or depleted (-C) hamsters, P ≤ 0.05.