Transphosphatidylation reaction products obtained using sunflower PLD. (A) TLC pattern of PEt and PA formation. Incubation conditions and lipid chromatography are described in Materials and Methods. The arrows point to the PEt, PA, and the origin of application. (B) Substrate specificities of sunflower and soybean cell PLDs. The final concentration of all the phospholipids used was 0.5 mM. PLD was assayed (0.5 ml total volume) by measuring the PEt formation as described in Materials and Methods. Each assay was carried out with 5 μg of sunflower or soybean cell PLD in the presence of 150 mM ethanol (final concentration) containing 0.5 μCi of [14C]ethanol. Results are shown as means from two independent experiments.