Table 1 Primers and fluorescent probes for real-time RT-PCR
Gene | Forward primer | Reverse primer | Probe |
|---|---|---|---|
GAPDH | ggaaggtgaaggtcggagtc | cgttctcagccttgacggt | 5'-FAM-tttggtcgtattgggcgcctg-TAMRA |
ApoM | tgccccggaaatggatcta | cagggcggccttcagtt | 5'-FAM-cacctgactgaagggagcacagatctca-TAMRA |
ApoA1 | ctgggataacctggaaaaggagac | ggaagtcgtccaggtagggct | 5'-FAM-agatgagcaaggatctggaggaggtgaa-TAMRA |
SHP1 | aaagggaccatcctcttcaacc | agggttccaggacttcacacag | 5'-FAM-cctccaagccgcctcccacatt-TAMRA |
LRH-1 | ttagtggcaaaacttcgttctctcc | agggcggcattgacttgttc | 5'-FAM-agttcgtatgtctgaaattcttggtgctct-TAMRA |
ABCA1 | caaggggtaggagaaagagacgc | ctcagccagcacccccag | 5'-FAM-ccagccacggcgtccctgctgt-TAMRA |