Figure 3
From: HBV inhibits apoB production via the suppression of MTP expression
ApoB mRNA and protein expression in HepG2 cells transfected with pHBV1.3 and HepG2 cells transfected with pBlue-ks assessed by RT-PCR and Western blot, respectively. (A) RT-PCR analysis was used to compare the relative levels of apoB mRNA in HepG2 cells transfected with pHBV1.3 and HepG2 cells transfected with pBlue-ks. β-actin gene was amplified as an internal control. PCR products were detected on 2% agarose gel electrophoresis with ethidium bromide stain.(B) Western blot analysis of apoB protein expression in HepG2 cells transfected with pHBV1.3 and HepG2 cells transfected with pBlue-ks. Protein samples from whole cell extracts were fractionated on 12% SDS-PAGE and then subjected to Western blot using a specific antibody to apoB. β-actin was the internal control.