Figure 4
From: HBV inhibits apoB production via the suppression of MTP expression
MTP mRNA and protein expression in HepG2 cells transfected with different amounts of pHBV1.3. (A) RT-PCR analysis was used to compare the relative levels of MTP mRNA in HepG2 cells transfected with 0 μg, 0.5 μg, 1 μg, and 2 μg pHBV1.3. β-actin gene was amplified as an internal control. PCR products were detected on 2% agarose gel electrophoresis with ethidium bromide stain.(B) Western blot analysis of MTP protein expression in HepG2 cells transfected with 0 μg, 0.5 μg, 1 μg, and 2 μg pHBV1.3. Protein samples from whole cell extracts were fractionated on 12% SDS-PAGE and then subjected to Western blot using a specific antibody to MTP. β-actin was the internal control.