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Figure 1 | Lipids in Health and Disease

Figure 1

From: Fish polar lipids retard atherosclerosis in rabbits by down-regulating PAF biosynthesis and up-regulating PAF catabolism

Figure 1

Representative HPLC chromatograph of GSBPL. Mobile phase was composed of solvent A; acetonitrile/methanol (60:40 v/v), solvent B; methanol (100%) and solvent C; water (100%). The mobile phase flow rate was adjusted to 1.0 mL/min, and UV detection wavelength was set at 208 nm. After 55 min holding of 100% solvent A, a linear ramp up to 100% B over 5 min was developed, followed by a 10 min holding of 100% B, then a linear ramp up to 100% C over 5 min was followed by a 30 min holding of 100% C. A 35 min equilibration was performed before the next analysis. Elution times of standard polar lipids were the following: phospahtidylcholine (PC) at 50-60 min, sphingomyelin (SM) at 62-67 min, lysophospahtidylcholine (LPC) at 68-80 min and digalactosyldiglycerides DGDG at 81-94 min.

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Lipids in Health and Disease

ISSN: 1476-511X