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Table 2 PAF metabolic enzyme specific activities in plasma, leukocytes and platelets along with PAF levels in rabbit blood upon experimental diet.

From: Fish polar lipids retard atherosclerosis in rabbits by down-regulating PAF biosynthesis and up-regulating PAF catabolism

Biochemical parameter

Time (days)

A (n = 6)

B(n = 6)

PAF-CPT specific activity in Leukocytes

(nmol PAF/min/mg protein)

0

0.85 ± 0.29

0.57 ± 0.36

 

45

2.52 ± 0.81a

0.66 ± 0.51b

PAF-CPT specific activity in platelets

(nmol PAF/min/mg protein)

0

2.04 ± 1.11

1.49 ± 0.80

 

45

1.89 ± 1.30

0.49 ± 0.31a

Lyso PAF AT specific activity in Leukocytes

(nmol PAF/min/mg protein)

0

0.0545 ± 0.0427

0.0200 ± 0.0149

 

45

0.1102 ± 0.0517

0.0256 ± 0.0239b

Lyso PAF AT specific activity in platelets

(nmol PAF/min/mg protein)

0

0.0047 ± 0.0023

0.0042 ± 0.0030

 

45

0.0029 ± 0.0022

0.0011 ± 0.0006a

PAFAH specific activity in Leukocytes

(nmol PAF/min/mg protein)

0

340 ± 61.3

273 ± 109

 

45

163 ± 15.7a

581 ± 340b

PAFAH specific activity in Platelets

(nmol PAF/min/mg protein)

0

96.5 ± 30.4

112 ± 20.2

 

45

25.0 ± 7.62a

30.8 ± 10.7a

Lp-PLA2 specific activity in plasma

(pmol PAF/min/μL PPP)

0

128 ± 35.3

109 ± 15.2

 

45

174 ± 21.6a

239 ± 56.7a

PAF levels in plasma; Free PAF levels

(pM)

0

3.1 ± 1.7

3.04 ± 1.23

 

45

5.0 ± 1.8a

0.24 ± 0.12a,b

PAF levels in cells; Bound PAF levels

(pM)

0

0.88 ± 0.15

1.08 ± 0.36

 

45

1.8 ± 0.34a

0.18 ± 0.08a,b

  1. Group A followed a 1% cholesterol atherogenic diet, while group B followed the atherogenic diet supplemented with 0,06% (w/w) Gilthead Sea Bream (Sparus aurata) Polar Lipids (GSBPL). Results are expressed as Mean Values ± Standard Deviation. a: Denotes statistically significant difference within the same group (day 45 vs day 0; p < 0.05), according to paired t-test. b: Denotes statistically significant difference between groups A and B (at 0 or 45 day; p < 0.05), according to one-way ANOVA test.